吉林地区奶源金黄色葡萄球菌耐药性及遗传特征分析  

Analysis of antibiotic resistance and genetic characteristics of milk derived Staphylococcus aureus in Jilin

作　　者：刘倩宏 马鹏明 顾巧逸 杨闻 李春花 LIU Qian-Hong;MA Peng-Ming;GU Qiao-Yi;YANG Wen;LI Chun-Hua(College of Animal Science,Xichang University,Xichang 615000,China;Xichang Agriculture and Rural Bureau,Xichang 615000,China;College of Animal Science and Technology,Jilin Agricultural Science and Technology University,Jilin 132101,China)

机构地区：[1]西昌学院动物科学学院,西昌615000 [2]西昌市农业农村局,西昌615000 [3]吉林农业科技学院动物科技学院,吉林132101

出　　处：《食品安全质量检测学报》2024年第14期317-324,共8页Journal of Food Safety and Quality

基　　金：吉林省自然科学基金项目(YDZJ202101ZYTS101);西昌学院博士科研启动项目(YBZ202256)。

摘　　要：目的了解鲜牛乳中金黄色葡萄球菌(Staphylococcus aureus,S.aureus)流行情况及耐药情况、分子特征和遗传特征。方法细菌分离培养及聚合酶链式反应(polymerase chain reaction,PCR)鉴定S.aureus,K-B药敏纸片扩散法检测其耐药性,对其中44个分离株进行全基因组测序(whole genomic sequence,WGS),并分析分子特征及遗传特性等。结果共分离出214株S.aureus,对13种常用抗生素均有耐药性,对青霉素产生耐药性菌株最多(145/214,67.76%)。44个流行株中,耐药表型达31种,25个菌株有多重耐药;检测到β-内酰胺类、四环素类等12类耐药基因及季铵盐类消毒剂耐药基因,其中6株检测到耐利奈唑胺基因;L8株携带11种毒力基因,至少90%的流行株携带aur、hlgA/B/C基因毒力;ST9-t899是流行优势型。SNP-WGS全基因组遗传进化分析,提示有人、畜间互传可能性。结论吉林地区牛场样本中S.aureus流行率为42.80%,存在多重耐药,且耐药谱广、耐药表型多样;有人畜间传播可能。鲜牛乳作为初级食品,对其中S.aureus特别是耐甲氧西林金黄色葡萄球菌耐药性及分子特征监测,是对食品卫生及公共卫生安全的重要保障。Objective To explore the prevalence,antimicrobial resistance phenotype and genotype,molecular and genetic characteristics of Staphylococcus aureus(S.aureus)in fresh milk.Methods Bacterial isolation and polymerase chain reaction(PCR)were used to identify S.aureus,K-B drug sensitive disk diffusion were used to detect drug resistance of isolated strains,then 44 isolated strains were used for whole genome sequencing to analyze their molecular and genetic characteristics.Results A total of 214 strains of S.aureus were isolated,which were resistant to 13 kinds of common antibiotics,and the most resistant strains were penicillin(145/214,67.76%).Among the 44 endemic strains,31 types of drug-resistant phenotypes were found,and 25 strains had multiple drug resistance.The 12 kinds of drug resistance genes such asβ-lactam,tetracycline and quaternary ammonium salt disinfectant were detected,and linezolid resistance genes were detected in 6 strains.L8 strain carried with 11 virulence genes,and at least 90%of strains carried aur and hlgA/B/C virulence genes;ST9-t899 was a dominant type.SNPs-WGS suggested the possibility of transmission between humans and animals.Conclusion The prevalence of S.aureus in cattle farm in Jilin is 42.80%,with multiple drug resistance,wide drug resistance spectrum and diverse drug resistance phenotype;and human-to-animal transmission is possible.As a primary food,monitoring the antimicrobial resistance and molecular characteristics of S.aureus,especially methicillin-resistant S.aureus,in fresh milk is an important guarantee for food hygiene and public health safety.

关 键 词：鲜牛乳 金黄色葡萄球菌 耐药性 遗传特征 全基因组测序 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]