鮰鱼骨胶原蛋白肽-钙螯合物促进钙转运和成骨细胞分化作用  

Promotive Effect of Catfish Bone Collagen Peptide-Calcium Chelate on Calcium Transport and Osteoblast Differentiation

作　　者：黄春林 林文静 谢星 汤林怡 温庆辉[1,2] 王乐怀 熊含露 张露[1,2] 涂宗财[1,3] HUANG Chunlin;LIN Wenjing;XIE Xing;TANG Linyi;WEN Qinghui;WANG Lehuai;XIONG Hanlu;ZHANG Lu;TU Zongcai(College of Health,College of Life Sciences,Jiangxi Normal University,Nanchang 330022,China;National R&D Center for Freshwater Fish Processing,Jiangxi Normal University,Nanchang 330022,China;State Key Laboratory of Food Science and Resources,Nanchang University,Nanchang 330047,China)

机构地区：[1]江西师范大学健康学院,生命科学学院,江西南昌330022 [2]江西师范大学国家淡水鱼加工技术研发专业中心,江西南昌330022 [3]南昌大学食品科学与资源挖掘全国重点实验室,江西南昌330047

出　　处：《食品科学》2024年第17期35-41,共7页Food Science

基　　金：江西省青年科学基金项目(20224BAB215051);江西省教育厅项目(GJJ2200382)。

摘　　要：以实验室前期制备的鮰鱼骨胶原蛋白肽-钙螯合物(F3-Ca螯合物)为原料,采用Caco-2细胞模型评价其对钙转运和吸收的影响,再分析其对hFoB1.19成骨细胞碱性磷酸酶(alkaline phosphatase,ALP)活性以及分化关键基因和蛋白表达的影响。结果发现F3-Ca螯合物能使Caco-2细胞的钙转运量提高到0.31 mg/mL,并促进成骨细胞的矿化。当F3-Ca螯合物的质量浓度为100μg/mL时,成骨细胞ALP的活性提高了53.68%。反转录聚合酶链式反应和蛋白质免疫印迹分析表明F3-Ca螯合物能提高成骨细胞中骨保护素(osteoprotegerin,OPG)、骨钙素和Runt相关转录因子2的基因和蛋白表达水平,降低核因子受体激活因子-κB配体(receptor activator for nuclear factor-κB ligand,RANKL)的基因和蛋白表达水平,增大OPG/RANKL的比值,从而激活OPG/RANKL/核因子受体激活因子-κB信号通路,促进成骨细胞的增殖、分化和矿化,达到加强钙吸收和抗骨质疏松的作用。本研究可为鮰鱼骨的高值化利用和新型钙补充剂的开发提供科学依据。Catfish bone collagen peptide-calcium(F3-Ca)chelatate,which had been prepared in our laboratory,was evaluated for its effect on calcium transport and absorption using a Caco-2 cell model.Its effect on the alkaline phosphatase(ALP)activity of osteoblast hFoB1.19 and the expression of the key genes and proteins involved in osteoblast differentiation was also investigated.The results indicated that the chelate increased calcium transport in Caco-2 cells to 0.31 mg/mL,and promoted the mineralization of osteoblasts.The chelate at a concentration of 100μg/mL increased the ALP activity of osteoblasts by 53.68%compared with the control group.Reverse transcription-polymerase chain reaction(RTPCR)and protein immunoblot analysis showed that F3-Ca chelate increased the gene and protein expression levels of osteoprotegerin(OPG),osteocalcin(OCN)and Runt-related transcription factor 2(RUNX2)in osteoblasts,reduced those of receptor activator for nuclear factor-κB ligand(RANKL),and increase the ratio of OPG/RANKL,thus activating the OPG/RANKL/receptor activator for nuclear factor-κB(RANK)signaling pathway,promoting the proliferation,differentiation and mineralization of osteoblasts,and ultimately resulting in enhanced calcium absorption and anti-osteoporosis activity.This study provides a scientific basis for the high-value utilization of catfish bone and the development of new calcium supplements.

关 键 词：鮰鱼骨胶原蛋白肽-钙螯合物 钙吸收 成骨细胞分化 骨质疏松症 钙转运 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]