近冰温贮藏在延缓甜龙竹笋采后木质化衰老中的作用  

Effect of Near-Freezing Temperature Storage on Delaying Postharvest Lignification of Dendrocalamus brandisii Shoots

作　　者：杨双鹤 申挥 罗海波 苏火生 邹琴 俞源河 陈亚男 余元善[4] 胡腾根 董霞[1] 于丽娟 YANG Shuanghe;SHEN Hui;LUO Haibo;SU Huosheng;ZOU Qin;YU Yuanhe;CHEN Yanan;YU Yuanshan;HU Tenggen;DONG Xia;YU Lijuan(College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,China;Agro-products Processing Research Institute,Yunnan Academy of Agricultural Sciences,Kunming 650233,China;School of Food Science and Pharmaceutical Engineering,Nanjing Normal University,Nanjing 210023,China;Sericultural&Agri-food Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510610,China)

机构地区：[1]云南农业大学食品科学技术学院,云南昆明650201 [2]云南省农业科学院农产品加工研究所,云南昆明650233 [3]南京师范大学食品与制药工程学院,江苏南京210023 [4]广东省农业科学院蚕业与农产品加工研究所,广东广州510610

出　　处：《食品科学》2024年第17期216-225,共10页Food Science

基　　金：云南省重大科技专项(202202AE090023)。

摘　　要：以室温和4℃为对照,以勃氏甜龙竹笋(Dendrocalamus brandisii)为研究对象,通过分析贮藏过程中呼吸强度、质量损失率、硬度、H2O2含量、木质素含量、木质素合成关键酶活性和木质素合成关键基因表达情况,总结近冰温(near-freezing temperature,NFT)贮藏对竹笋采后木质化的调控作用。结果表明:相较于室温和4℃,NFT贮藏显著抑制了甜龙竹笋呼吸强度、质量损失率、硬度、H2O2含量、木质素含量上升;延缓了苯丙氨酸解氨酶、肉桂醇脱氢酶、肉桂酸羟化酶和氧化物酶活性升高;同时基于转录组技术发现PAL、4CL、HCT、CCR、CAD等家族基因中有33个基因表达量下调,25个基因表达量上调;相关性分析进一步表明大部分生理指标、木质素合成关键酶活性和关键基因表达情况与木质素含量呈显著正相关。综上,NFT贮藏可通过影响呼吸强度、质量损失率、硬度、H2O2含量、木质素含量、木质素合成关键酶活性和合成关键基因表达,有效延缓甜龙竹笋采后木质化衰老。In this study,the regulatory effect of near-freezing temperature(NFT)storage on postharvest lignification of Dendrocalamus brandisii shoots was evaluated.Samples stored at room temperature and 4℃served as controls.Respiratory intensity,mass loss rate,hardness,H2O2 content,lignin content,and the activities and gene expression of key enzymes involved in lignin synthesis were measured during storage.The results showed that compared with the controls,NFT storage significantly inhibited the increase of respiration intensity,mass loss,hardness,H2O2 content and lignin content in D.brandisii shoots,and delayed the increase of phenylalanine ammonia-lyase(PAL),cinnamyl alcohol dehydrogenase(CAD),cinnamate 4-hydroxylase(C4H),and peroxidase(POD)activities.Transcriptomic analysis showed that 33 genes members in PAL,4CL,HCT,CCR,and CAD families were down-regulated and 25 gene members were up-regulated.Correlation analysis revealed that most physiological indexes and the activities and gene expression of key enzymes involved in lignin synthesis were significantly and positively correlated with the lignin content.In conclusion,NFT storage could effectively suppress the lignification of D.brandisii shoots during storage by regulating the respiratory intensity,mass loss rate,hardness,H2O2 content,lignin content,and key enzyme activities and gene expression related to lignin synthesis.

关 键 词：竹笋 近冰温贮藏 木质化 转录组 采后 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]