应城萝卜泡菜中产γ-氨基丁酸乳酸菌的分离纯化及其特性  

Isolation and Purification ofγ-Aminobutyric Acid Lactic Acid Bacteria from Yingcheng Radish Kimchi and Its Characteristics

作　　者：朱静[1] 陈晖 张青祥 陈亚蓝 刘海波 桑大席 陈龙 ZHU Jing;CHEN Hui;ZHANG Qingxiang;CHEN Yalan;LIU Haibo;SANG Daxi;CHEN Long(School of Food Science and Technology,Xinyang Agriculture and Forestry University,Xinyang 464000,China;College of Agricultural Engineering and Food Science,Shandong University of Technology,Zibo 255000,China)

机构地区：[1]信阳农林学院食品科学与工程学院,河南信阳464000 [2]山东理工大学农业工程与食品科学学院,山东淄博255000

出　　处：《食品科技》2024年第7期24-31,共8页Food Science and Technology

基　　金：河南省重点研发与推广专项(科技攻关)(212102110314);河南省高等学校重点科研项目(24B180016,24B550017);信阳农林学院高水平科研孵化器建设基金项目(FCL202014)。

摘　　要：从应城萝卜泡菜中筛选鉴定产γ-氨基丁酸(γ-Aminobutyric acid, GABA)的菌株并优化其发酵条件。通过稀释涂布平板法和划线分离法共筛选出45株乳酸菌,利用薄层色谱法定性复筛出7株产GABA乳酸菌,最后利用高效液相色谱法筛选出GABA产量较高的菌株LS-23,其产量可达1.039mg/m L。通过形态观察、生理生化特征试验以及16S rDNA序列同源性分析,可确定该菌株为植物乳杆菌。该菌株革兰氏染色阳性,接触酶和吲哚检验阴性,24 h时,发酵液酸度最低为3.565,对数生长期在4~18 h。以菌体生长量及发酵液GABA含量为依据,果糖和酵母膏是该菌株的最适碳源和氮源。该研究可为产GABA乳酸菌的产量优化奠定理论基础。Strains producing gamma-aminobutyric acid(GABA)are screened and identified from Yingcheng radish pickles and their fermentation conditions are optimized.45 strains of Lactobacillus were screened by dilution coated plate method and streak separation method,and 7 strains of GABA-producing bacteria were qualitatively screened by thin layer chromatography.The Lactobacillus LS-23 with high GABA yield was selected by high performance liquid chromatography,and its yield was 1.039 mg/mL.The strain was identified as Lactobacillus plantarum by morphological characteristics,physiological and biochemical characteristics and 16S rDNA sequence homology analysis.The strain was positive for Gram staining and negative for indole and contase tests.The lowest acidity of fermentation broth was 3.565 at 24 h,and the logarithmic growth stage was 4–18 h.Based on the growth amount of bacteria and the content of GABA in fermentation liquor,the optimal carbon source and nitrogen source of the strain were fructose and yeast paste,respectively.This study laid a theoretical foundation for the yield optimization of GABA-producing lactic acid bacteria.

关 键 词：Γ-氨基丁酸 植物乳杆菌 筛选 分子鉴定 生理生化试验 

分 类 号：TS201.3[轻工技术与工程—食品科学]