超声辅助离子液体法和酶解法提取鸡枞菌多糖及抗氧化性研究  

Study on Extraction of Polysaccharides from Collybia albuminosa by Ultrasonic-Assisted Ionic Liquid Method and Enzymatic Hydrolysis Method and Their Antioxidant Activity

作　　者：贾庆超 JIA Qing-chao(School of Food Science and Engineering,Zhengzhou University of Science and Technology,Zhengzhou 450064,China)

机构地区：[1]郑州科技学院食品科学与工程学院,郑州450064

出　　处：《中国调味品》2024年第9期79-88,共10页China Condiment

基　　金：河南省教育厅重点研究项目(24B550021);郑州科技学院2022年度校级科研项目(XJKY08)。

摘　　要：以鸡枞菌为原料,采用超声辅助离子液体法和酶解法提取鸡枞菌多糖,研究鸡枞菌多糖的最佳提取条件,并对提取的多糖进行抗氧化性研究。结果表明,超声辅助离子液体法提取多糖的最佳工艺为离子液体体积1.5 mL、超声时间33 min、料液比1∶33、超声温度56℃,在此条件下,多糖提取量为26.79 mg/g。超声辅助酶解法提取多糖的最佳工艺为酶溶液体积2.2 mL、提取温度51℃、料液比1∶21、提取时间51 min,在此条件下多糖提取量为18.67 mg/g。在两种方法的基础上探讨鸡枞菌多糖对DPPH·和·OH的清除能力,将其清除能力与V C作对比。结果表明,对DPPH·和·OH的清除能力均随着浓度的增大而增大,当鸡枞菌多糖浓度为6 mg/mL时,超声辅助离子液体法和超声辅助酶解法得到的多糖对DPPH·和·OH的清除能力均达到最大,但均比同等浓度的V C低,对·OH的清除能力分别为59.59%和51.78%;对DPPH·的清除能力分别为73.98%和62.87%,表明鸡枞菌多糖具有良好的抗氧化能力。该研究为鸡枞菌多糖的研究提供了可靠的理论依据。With Collybia albuminosa as the raw material,polysaccharides are extracted from Collybia albuminosa by ultrasonic-assisted ionic liquid method and enzymatic hydrolysis method.The optimal extraction conditions for polysacharides from Collybia albuminosa and antioxidant activity of the extracted polysaccharides are studied.The results show that the optimal process for extracting polysaccharides using ultrasound-assisted ionic liquid method is ionic liquid volume of 1.5 mL,ultrasonic time of 33 min,solid-liquid ratio of 1∶33 and ultrasonic temperature of 56℃.Under these conditions,the extraction amount of polysaccharides is 26.79 mg/g.The optimal process for extracting polysaccharides using ultrasonic-assisted enzymatic hydrolysis method are enzyme solution volume of 2.2 mL,extraction temperature of 51℃,solid-liquid ratio of 1∶21 and extraction time of 51 min.Under these conditions,the extraction amount of polysaccharides is 18.67 mg/g.On the basis of the two methods,the scavenging capacity of Collybia albuminosa polysaccharides on DPPH·and·OH is investigated,and their scavenging capacity is compared with that of V C.The results show that the scavenging capacity on DPPH·and·OH increases with the increase of concentration.When the concentration of Collybia albuminosa polysaccharides is 6 mg/mL,the scavenging capacity of the polysaccharides extracted by ultrasonic-assisted ionic liquid method and ultrasonic-assisted enzymatic hydrolysis method on DPPH·and·OH both reaches the highest,which is lower than that of V C at the same concentration.The scavenging capacity on·OH is 59.59%and 51.78%respectively,and the scavenging capacity on DPPH·is 73.98%and 62.87%respectively,indicating that the polysaccharides from Collybia albuminosa have good antioxidant capacity.This study has provided a reliable theoretical basis for the study on Collybia albuminosa polysaccharides.

关 键 词：鸡枞菌 多糖 超声辅助离子液体法 超声辅助酶解法 抗氧化性 

分 类 号：TS201.2[轻工技术与工程—食品科学]