吡咯伯克霍尔德氏菌JK-SH007产脂肪酶发酵条件优化及酶学特性  

Fermentation optimization and enzymatic characterization oflipase-producing antagonistic bacteria Burkholderia pyrrocinia JK-SH007

作　　者：白凤麟 赵波 叶建仁 任嘉红[1] BAI Fenglin;ZHAO Bo;YE Jianren;REN Jiahong(Department of Life Sciences,Changzhi College,Changzhi,Shanxi Province 046011,China;College of Forestry,Nanjing forestry University,Nanjing,Jiangsu Province 210037,China)

机构地区：[1]长治学院生命科学系,山西长治046011 [2]南京林业大学林学院,江苏南京210037

出　　处：《中国饲料》2024年第17期32-39,共8页China Feed

基　　金：山西省“1331工程”重点学科建设(1331KSC);国家级大学生创新创业训练计划项目(20221124);国家自然科学基金面上项目(32071770)。

摘　　要：为提高内生生防菌吡咯伯克霍尔德氏菌JK-SH007发酵产脂肪酶的能力,并确定该菌株脂肪酶的酶学性质,通过单因子试验和正交设计试验,优化该菌株产脂肪酶的发酵培养条件,初步考察了温度、pH、金属离子和有机溶剂对其活性的影响。结果显示:JK-SH007菌株产脂肪酶最优培养基组成及发酵条件为:葡萄糖15 g/L,蛋白胨20 g/L,K2HPO42.5 g/L,花生油400μL/50 mL,初始pH 8.0,发酵温度33℃。该脂肪酶反应最适温度为60℃,且在50~60℃条件下具有良好热稳定性;反应最适pH为8.0,在pH 5.0~10.0活性稳定;该脂肪酶对甲醇、乙醇、乙酸乙酯的耐受性好,具有较高甲醇抗性。研究结果表明,该酶对温度和pH均具有较宽的适应度,具有开发成生物催化剂的潜力。To improve the production of lipase from endophytic antagonistic bacteria Burkholderia pyrrocinia JK-SH007 and analyze its enzymatic properties,single factor test and orthogonal design method were performed to determine the optimal conditions of lipase-producing for strain JK-SH007.The impact of temperature,pH and organic solvents on lipase catalytic reaction was also investigated.The results showed that the optimum operating conditions were found as glucose 15 g/L,peptone 20 g/L,K2HPO42.5 g/L,peanut oil 400μL/50mL,at pH 8.0 and 33℃.The optimal temperature and pH were 60℃and 9.0,respectively.The lipase was stable between temperature 50~60℃and pH 5.0~10.0.Moreover,the lipase had solid tolerance to methanol,ethanol and ethyl acetate,especially to methanol.The lipase had a wider fitness for temperature,pH and possess favorable characteristics to meets the basic requirements of biodiesel catalyst.This work provided important clues for the exploitation and utilization of JK-SH007 in environment management and industrial production.

关 键 词：脂肪酶 吡咯伯克霍尔德氏菌 发酵条件 酶学性质 

分 类 号：S816.3[农业科学—饲料科学]