Aurora B promotes the CENP-T–CENP-W interaction to guide accurate chromosome segregation in mitosis  

作　　者：Wei Liu Zhen Dou Chunyue Wang Gangyin Zhao Fengge Wu Chunli Wang Felix Aikhionbare Mingliang Ye Divine Mensah Sedzro Zhenye Yang Chuanhai Fu Zhikai Wang Xinjiao Gao Xuebiao Yao Xiaoyu Song Xing Liu 

机构地区：[1]MOE Key Laboratory for Cellular Dynamics,Center for Advanced Interdisciplinary Science and Biomedicine of IHM,University of Science and Technology of China School of Life Sciences,Hefei 230027,China [2]Keck Center for Cellular Dynamics and Organoids Plasticity,Atlanta,GA 30310,USA [3]Anhui Key Laboratory for Cellular Dynamics and Chemical Biology,Hefei National Research Center for Interdisciplinary Sciences at the Microscale,Hefei 230026,China [4]National Chromatographic Research and Analysis Center,Chinese Academy of Sciences,Dalian 116023,China

出　　处：《Journal of Molecular Cell Biology》2024年第2期11-23,共13页分子细胞生物学报（英文版）

基　　金：supported by the National Key Research and Development Program of China(2022YFA1303100,2022YFA0806800,2022YFA1302700,and 2017YFA0503600);the National Natural Science Foundation of China(32090040,92254302,92153302,92253301,22137007,32170733,and 31871359);the Ministry of Education(IRT_17R102);the Plans for Major Provincial Science&Technology Projects of Anhui Province(202303a0702003);the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB19040000);the Fundamental Research Funds for the Central Universities(WK2070000066 and WK2070000194).

摘　　要：Accurate chromosome segregation in mitosis depends on kinetochores that connect centromeric chromatin to spindle microtubules.Centromeres are captured by individual microtubules via a kinetochore constitutive centromere-associated network(CCAN)during chromosome segregation.CCAN contains 16 subunits,including CENP-W and CENP-T.However,the molecular recognition and mitotic regulation of the CCAN assembly remain elusive.Here,we revealed that CENP-W binds to the histone fold domain and an uncharacterized N-terminal region of CENP-T.Aurora B phosphorylates CENP-W at threonine 60,which enhances the interaction between CENP-W and CENP-T to ensure robust metaphase chromosome alignment and accurate chromosome segregation in mitosis.These findings delineate a conserved signaling cascade that integrates protein phosphorylation with CCAN integrity for the maintenance of genomic stability.

关 键 词：MITOSIS KINETOCHORE Aurora B CENP-T CENP-W PHOSPHORYLATION 

分 类 号：Q343.2[生物学—遗传学]