PLK1 phosphorylation of ZW10 guides accurate chromosome segregation in mitosis  

作　　者：Sm Faysal Bellah Fangyuan Xiong Zhen Dou Fengrui Yang Xing Liu Xuebiao Yao Xinjiao Gao Liangyu Zhang 

机构地区：[1]MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics,Hefei National Research Center for Physical Sciences at the Microscale,University of Science and Technology of China School of Life Sciences,Hefei 230027,China [2]Anhui Key Laboratory for Cellular Dynamics and Chemical Biology,Hefei 230027,China

出　　处：《Journal of Molecular Cell Biology》2024年第2期47-62,共16页分子细胞生物学报（英文版）

基　　金：supported by grants from the Ministry of Science and Technology of China and the National Natural Science Foundation of China(2022YFA1303100,32090040,92254302,2022YFA0806800,91854203,31621002,2017YFA0503600,21922706,and 92153302 to X.L.;92053104 to X.G.);the Plans for Major Provincial Science&Technology Projects of Anhui Province(202303a0702003 to X.L.);the Ministry of Education(IRT_17R102 to X.L.);the Fundamental Research Funds for the Central Universities(KB9100000007 and KB9100000013 to X.L.)。

摘　　要：Stable transmission of genetic information during cell division requires faithful chromosome segregation.Mounting evidence has demonstrated that polo-like kinase 1(PLK1)dynamics at kinetochores control correct kinetochore–microtubule attachments and subsequent silencing of the spindle assembly checkpoint.However,the mechanisms underlying PLK1-mediated silencing of the spindle checkpoint remain elusive.Here,we identified a regulatory mechanism by which PLK1-elicited zeste white 10(ZW10)phosphorylation regulates spindle checkpoint silencing in mitosis.ZW10 is a cognate substrate of PLK1,and the phosphorylation of ZW10 at Ser12 enables dynamic ZW10–Zwint1 interactions.Inhibition of ZW10 phosphorylation resulted in misaligned chromosomes,while persistent expression of phospho-mimicking ZW10 mutant caused premature anaphase,in which sister chromatids entangled as cells entered anaphase.These findings reveal the previously uncharacterized PLK1–ZW10 interaction through which dynamic phosphorylation of ZW10 fine-tunes accurate chromosome segregation in mitosis.

关 键 词：ZW10 PLK1 KINETOCHORE MITOSIS PHOSPHORYLATION 

分 类 号：Q253[生物学—细胞生物学]