宁海白和大房枇杷F_(1)代叶、花和果抗氧化活性分析与评价  

Analysis and evaluation of antioxidant activity of leaves,flowers and fruits of loquat F_(1)generation of Ninghaibai and Oobusa

作　　者：朱启轩 李晓颖[1] 葛航 武军凯 王志轩 陈俊伟[1] 徐红霞[1] ZHU Qixuan;LI Xiaoying;GE Hang;WU Junkai;WANG Zhixuan;CHEN Junwei;XU Hongxia(Institute of Horticulture,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,Zhejiang,China;College of Horticulture Science&Technology,Hebei Normal University of Science&Technology,Qinhuangdao 066600,Hebei,China;Hebei Key Laboratory of Horticultural Germplasm Excavation and Innovative Utilization,Qinhuangdao 066600,Hebei,China)

机构地区：[1]浙江省农业科学院园艺研究所,杭州310021 [2]河北科技师范学院园艺科技学院,河北秦皇岛066600 [3]河北省特色园艺种质挖掘与创新利用重点实验室,河北秦皇岛066600

出　　处：《果树学报》2024年第9期1716-1730,共15页Journal of Fruit Science

基　　金：浙江省“十四五”果品新品种选育专项(2021C02066-3);浙江省三农九方项目(2022SNJF028)。

摘　　要：【目的】探讨枇杷F_(1)代的叶、花和果中总酚、类黄酮含量及抗氧化活性的多样性,以期为枇杷新品种选育与功能性物质成分开发利用提供参考。【方法】利用Folin-Ciocalteu法、亚硝酸钠-三氯化铝法、DPPH(1,1-二苯基-2-苦基苯肼)法、ABTS[2,2’-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐]法、FRAP(铁离子还原能力)法测定亲本宁海白(Ninghaibai)和大房(Oobusa)及其29个杂交F_(1)代的叶、花、果中总酚含量、类黄酮含量和体外抗氧化活性,并对其进行多样性分析、相关性分析、聚类分析以及抗氧化活性综合评价。【结果】F_(1)代的叶、花、果中总酚含量、类黄酮含量和抗氧化活性变异系数为9.51%~49.48%,除花的总酚含量外,其余各性状分离广泛并且叶中性状的遗传变异相较花、果更为丰富。此外,总酚含量、类黄酮含量和抗氧化活性在杂交后代不同器官中的含量存在较大差异,主要表现为花>叶>果。相关性分析表明,叶、果中的总酚、类黄酮含量与抗氧化活性呈极显著相关,花中的总酚、类黄酮含量与抗氧化活性具有一定相关性,但性状在叶、花、果等不同器官间无明显相关性。可通过APC(antioxidant potency composite)指数与聚类分析在杂交后代中筛选出抗氧化活性较强的株系。【结论】枇杷杂交F_(1)代的总酚、类黄酮含量以及抗氧化活性存在丰富的多样性,总酚和类黄酮是枇杷叶、花、果中重要抗氧化活性成分,总酚、类黄酮以及抗氧化活性在不同器官间无明显相关性。研究结果可为高抗氧化活性枇杷新品种的选育提供理论依据,也可为枇杷抗氧化物质的开发利用提供参考。【Objective】Loquat is native to China,and has a long history of cultivation and medicinal use.Loquat phenolic substances,as natural antioxidants,have a variety of biological activities.At present,most studies on loquat phenolic substances and antioxidant activity only focus on single organ such as leaf,flower,and fruit,and lack comparative analysis among the different organs.In addition,the evaluation of the antioxidant activity of loquat mainly involves ranking and evaluating the antioxidant activities measured by different methods,and there are few studies on comprehensive evaluation.In order to provide a theoretical basis for the breeding of new loquat varieties with high total phenolic,flavonoids content and high antioxidant activity,as well as to supply reference for the effectively developing and utilizing of loquat functional ingredients,this study aimed to explore the diversity of total phenolic,flavonoids contents and antioxidant activity in different organs of the loquat F_(1)hybrid population,and to comprehensively evaluate the antioxidant activity.【Methods】The total phenolic,flavonoids content and antioxidant activity of the different organs of Ninghaibai and Oobusa and their 29 F_(1)offsprings were determined.The total phenolics content was determined by Folin-ciocalteu method,the flavonoids content was determined by sodium nitrite-aluminum chloride method.DPPH(1,1-Diphenyl-2-picrylhydrazyl)radical scavenging capacity(DPPH value),ABTS(2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid)cation radical scavenging capacity(ABTS value),Ferric reducing antioxidant power(FRAP value)in vitro were determined by DPPH method,ABTS method and FRAP method,respectively.Diversity analysis and correlation analysis were conducted on the total phenolics,flavonoids and antioxidant activity of different organs of the hybrid offspring.In addition,a comprehensive evaluation and cluster analysis were conducted on the antioxidant activity of the hybrid progeny.【Results】There was a rich diversity in the total ph

关 键 词：枇杷 总酚 类黄酮 抗氧化活性 相关性 

分 类 号：S667.3[农业科学—果树学]