刺参总皂苷提取工艺及其抗氧化活性的研究  

Optimization of extraction process of total saponins from Apostichopus japonicus sea cucumber and its antioxidant activity

作　　者：刘芳[1] 贾爱荣 蔡英英 鞠文明 张绵松 LIU Fang;JIA Airong;CAI Yingying;JU Wenming;ZHANG Miansong(Biology Institute,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250103;China-Australia Joint Laboratory for Native Bioresource Industry Innovation,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250103;Weihai Haixiang Food Testing Service Co.,Ltd.,Weihai 264305;Weihai Homey Group Co.,Ltd.,Weihai 264305)

机构地区：[1]齐鲁工业大学(山东省科学院),山东省科学院生物研究所,济南250103 [2]中澳特色生物资源产业技术创新联合实验室,济南250103 [3]威海海翔食品检测服务有限公司,威海264305 [4]好当家集团有限公司,威海264305

出　　处：《中国食品添加剂》2024年第9期10-17,共8页China Food Additives

基　　金：山东省重点研发计划(2021TZXD008);山东省科技型中小企业创新能力提升工程项目(2022TSGC1306)。

摘　　要：为了探究刺参中总皂苷的最佳提取工艺和抗氧化能力,以总皂苷得率、总糖含量、蛋白质含量为指标,以乙醇浓度、料液比、提取温度和提取时间开展单因素试验,在单因素试验基础上,采用Box-Behnken响应面试验对总皂苷得率和蛋白质含量进行分析,同时对总皂苷的抗氧化能力进行检测。实验结果表明,在乙醇浓度91%、料液比(1∶31)g/mL、提取温度56℃、提取时间90 min的条件下,总皂苷得率为0.117%、蛋白质含量为8.078 mg/g,该条件下的总糖含量为5.808 mg/g。抗氧化实验结果表明,刺参总皂苷对DPPH自由基和ABTS自由基具有较好的清除作用,其质量浓度与两种自由基清除率呈现较好的相关性。研究结果为刺参总皂苷的提取及其进一步开发利用提供了理论参考。To optimize the extraction process and assess antioxidant capacity of total saponins from Apostichopus japonicus sea cucumber,single factor experiments were conducted.The ethanol concentration,solid-liquid ratio,extraction temperature and extraction time were used as factors,with the yield of total saponins,total sugar and protein content set as response variables.Based on single factor experiments,Box-Behnken response surface method was employed to further optimize the extraction process.The optimized process conditions,as determined by antioxidant activity experiments,were as follows:91%ethanol concentration,(1∶31)g/mL solid-liquid ration,56℃extraction temperature and 90 min extraction time.Under these conditions,the yield of total saponins from Apostichopus japonicus sea cucumber was 0.117%,with a protein content of 8.078 mg/g and the total sugar content 5.808 mg/g.Antioxidant assays showed that the total saponins from Apostichopus japonicus sea cucumber had strong scavenging effect against both DPPH and ABTS free radical,with a clear correlation between concentration and scavenging rates for both radicals.This study provides a valuable theoretical foundation for the extraction of total saponins from Apostichopus japonicus sea cucumber and their potential development and utilization in various applications.

关 键 词：刺参 总皂苷 响应面法 提取工艺 抗氧化活性 

分 类 号：TS202.1[轻工技术与工程—食品科学] TS254.9[轻工技术与工程—食品科学与工程]