Dioscin from Polygonatum sibiricum induces apoptosis and autophagy in Ishikawa human endometrial cancer cell and in vivo  

作　　者：Xiaoli Li Runhui Ma Zhijing Ni Wei Wang Kiran Thakur Jianguo Zhang Zhaojun Wei 

机构地区：[1]School of Biological Science and Engineering,North Minzu University,Yinchuan 750021,China [2]School of Food and Biological Engineering,Hefei University of Technology,Hefei 230009,China [3]School of Public Health,Anhui University of Science and Technology,Huainan 232001,China

出　　处：《Food Science and Human Wellness》2024年第5期2601-2616,共16页食品科学与人类健康（英文）

基　　金：supported by the National Key Research&Development Program of China(2022YFF1100305);the National Natural Science Foundation of Ningxia Province(2021AAC02019,2022AAC03230);the Key research and development projects in Ningxia province(2021BEF02013).

摘　　要：With an aim to comprehend the precise regulatory mechanism of dioscin against endometrial carcinoma(EC), we firstly extracted the components from Polygonatum sibiricum followed by identification and structural characterization. The anti-EC activity of dioscin was initially determined based on the inhibition of Ishikawa cell proliferation and tumor growth. The high-throughput sequencing data indicated that dioscin not only promoted apoptosis, including decrease of poly ADP-ribose polymerase(PARP) and B-cell lymphoma-2(Bcl-2) and increase of c-PARP and Bcl-2-associcated agonist of cell death(Bad), but also induced autophagy, including increase of autophagic lysosomes and LC3Ⅱ/LC3Ⅰ ratio. Mechanistic exploration suggested that dioscin induced autophagy and apoptosis through inhibition of PI3K/AKT/mTOR signaling pathway. Besides, the dioscin-regulated p53 pathway was mainly involved in autophagy induction. Furthermore, inhibition of Ishikawa cell autophagy was linked to dioscin-induced apoptosis. Our data suggest the immense potential of dioscin for the development of functional food for EC and related medical application.

关 键 词：DIOSCIN Ishikawa cell APOPTOSIS AUTOPHAGY PI3K/AKT p53 

分 类 号：TS201.4[轻工技术与工程—食品科学]