高效液相色谱法测定保健酒中淫羊藿苷的含量  

Determination of Icariin Content in Health Wine by High Performance Liquid Chromatography

作　　者：谢勇 吴凌 王天西 郑秋萍 居广琳 赵华楠 XIE Yong;WU Ling;WANG Tianxi;ZHENG Qiuping;JU Guanglin;ZHAO Huanan(China National Quality Supervision and Testing Center for Processed Food(Fuzhou),Fujian Inspection and Research Institute for Product Quality,Fuzhou 350002,China)

机构地区：[1]国家加工食品质量检验检测中心(福州),福建省产品质量检验研究院,福建福州350002

出　　处：《食品安全导刊》2024年第25期87-89,共3页China Food Safety Magazine

摘　　要：目的:建立高效液相色谱法测定保健酒中淫羊藿苷含量的分析方法。方法:保健酒中淫羊藿苷经甲醇提取,采用XB-C18色谱柱(150 mm×4.6 mm,5μm)分离,外标法定量。结果:淫羊藿苷在0.117~58.500μg·mL^(-1)质量浓度范围内呈良好的线性关系,相关系数大于0.999,方法检出限为0.1 mg·kg^(-1),定量限为0.3 mg·kg^(-1)。方法的加标回收率为95.3%~100.9%,相对标准偏差<5%。结论:该方法简便、灵敏、快速,可用于保健酒中淫羊藿苷的测定。Objective:To establish an analytical method for the determination of icariin content in health wine by high performance liquid chromatography.Method:Icariin in health wine was extracted by methanol,separated by XB-C18 column(150 mm×4.6 mm,5μm),and quantified by external standard method.Result:Icariin showed a good linear relationship in the mass concentration range of 0.117μg·mL^(-1) to 58.500μg·mL^(-1),the correlation coefficient was greater than 0.999,the detection limit of the method was 0.1 mg·kg^(-1),and the quantitative limit was 0.3 mg·kg^(-1).The recoveries of the spikes were 95.3%to 100.9%,and the relative standard deviation was less than 5%.Conclusion:This method is simple,sensitive and rapid,and can be used for the determination of icariin in health wine.

关 键 词：保健酒 淫羊藿苷 高效液相色谱法 

分 类 号：TS262.91[轻工技术与工程—发酵工程] O657.72[轻工技术与工程—食品科学与工程]