食品安全快速检测技术在食源性致病菌检测中的应用  

The Application of Fast Food Safety Detection Technology in the Detection of Foodborne Pathogens

作　　者：胡婵娟 胡莉 吴志豪 戚俊峰 HU Chanjuan;HU Li;WU Zhihao;QI Junfeng(School of Biological and Food Engineering,Huanghuai University,Zhumadian 463000,China;Department of General Medicine,Affiliated Zhumadian Central Hospital of Huanghuai University,Zhumadian 463000,China)

机构地区：[1]黄淮学院生物与食品工程学院,河南驻马店463000 [2]黄淮学院附属驻马店市中心医院全科医疗科,河南驻马店463000

出　　处：《食品安全导刊》2024年第15期162-164,168,共4页China Food Safety Magazine

基　　金：河南省重点研发专项项目(231111320300);河南省科技攻关项目(242102231059,232102310189);驻马店市科技创新青年专项项目(QNZX202319)。

摘　　要：本文针对食源性致病菌检测的迫切需求,深入分析了主要致病菌的特性,并探讨了食品安全快速检测技术的多种方法及其面临的挑战。文章重点介绍了核酸扩增、免疫分析、生物传感等技术的优势与局限,同时针对样品基质复杂性、低丰度致病菌的富集分离以及现场快速检测设备的稳定性问题,提出了具体的技术改进对策。通过优化检测技术,可显著提升食源性致病菌检测的灵敏度、特异性和操作便捷性,为食品安全监管提供强有力的技术支撑。This article focuses on the urgent need for the detection of foodborne pathogens,analyzes in depth the characteristics of the main pathogens,and explores various methods and challenges of rapid food safety detection technology.The article focuses on the advantages and limitations of technologies such as nucleic acid amplification,immunoassay,and biosensing.At the same time,specific technical improvement measures are proposed to address the complexity of sample matrices,the enrichment and separation of low abundance pathogenic bacteria,and the stability of on-site rapid detection equipment.By optimizing detection techniques,the sensitivity,specificity,and operational convenience of detecting foodborne pathogens can be significantly improved,providing strong technical support for food safety supervision.

关 键 词：食源性致病菌 快速检测 核酸扩增 

分 类 号：TS207.4[轻工技术与工程—食品科学] TS201.6[轻工技术与工程—食品科学与工程]