CNKSR2 interactome analysis indicates its association with the centrosome/microtubule system  

作　　者：Lin Yin Yalan Xu Jie Mu Yu Leng Lei Ma Yu Zheng Ruizhi Li Yin Wang Peifeng Li Hai Zhu Dong Wang Jing Li 

机构地区：[1]Institute for Translational Medicine,The Affiliated Hospital of Qingdao University,Medical College,Qingdao University,Qingdao,Shandong Province,China [2]School of Basic Medicine,Qingdao University,Qingdao,Shandong Province,China [3]College of Life Sciences,and School of Pharmacy,Medical College,Qingdao University,Qingdao,Shandong Province,China [4]Department of Urology,Qingdao Municipal Hospital Affiliated to Qingdao University,Qingdao,Shandong Province,China

出　　处：《Neural Regeneration Research》2025年第8期2420-2432,共13页中国神经再生研究（英文版）

基　　金：supported by the National Nature Science Foundation of China,No.32101020(to JL);the Natural Science Foundation of Shandong Province,Nos.ZR2020MC071(to JL),ZR2023MH327(to HZ);the Integrated Project of Major Research Plan of National Natural Science Foundation of China,No.92249303(to PL);the Natural Science Foundation of Qingdao,No.23-2-1-193-zyyd-jch(to HZ)。

摘　　要：The protein connector enhancer of kinase suppressor of Ras 2(CNKSR2),present in both the postsynaptic density and cytoplasm of neurons,is a scaffolding protein with several protein-binding domains.Variants of the CNKSR2 gene have been implicated in neurodevelopmental disorders,particularly intellectual disability,although the precise mechanism involved has not yet been fully understood.Research has demonstrated that CNKSR2 plays a role in facilitating the localization of postsynaptic density protein complexes to the membrane,thereby influencing synaptic signaling and the morphogenesis of dendritic spines.However,the function of CNKSR2 in the cytoplasm remains to be elucidated.In this study,we used immunoprecipitation and high-resolution liquid chromatography-mass spectrometry to identify the interactors of CNKSR2.Through a combination of bioinformatic analysis and cytological experiments,we found that the CNKSR2 interactors were significantly enriched in the proteome of the centrosome.We also showed that CNKSR2 interacted with the microtubule protein DYNC1H1 and with the centrosome marker CEP290.Subsequent colocalization analysis confirmed the centrosomal localization of CNKSR2.When we downregulated CNKSR2 expression in mouse neuroblastoma cells(Neuro 2A),we observed significant changes in the expression of numerous centrosomal genes.This manipulation also affected centrosome-related functions,including cell size and shape,cell proliferation,and motility.Furthermore,we found that CNKSR2 interactors were highly enriched in de novo variants associated with intellectual disability and autism spectrum disorder.Our findings establish a connection between CNKSR2 and the centrosome,and offer new insights into the underlying mechanisms of neurodevelopmental disorders.

关 键 词：autism spectrum disorder CENTROSOME CNKSR2 intellectual disability INTERACTOME mass spectrometry MICROTUBULE neurodevelopmental disease protein complexes protein-protein interactions 

分 类 号：Q421[生物学—神经生物学]