八月瓜果皮多糖提取工艺优化及其体外抗氧化、降糖活性分析  

Optimization of Polysaccharides Extraction from the Peels of Akebia trifoliate and Evaluation of Its in Vitro Antioxidant and Hypoglycemic Activity

作　　者：刘永玲[1] 刘向炼 陶欣 杨炜 许粟 LIU Yongling;LIU Xianglian;TAO Xin;YANG Wei;XU Su(College of Food Science and Engineering,Guiyang University,Guiyang 550005,China;Guizhou Engineering Research Center for Characteristic Flavor Perception and Quality Control of Drug-Food Homologous Resources,Guiyang 550005,China)

机构地区：[1]贵阳学院食品科学与工程学院,贵州贵阳550005 [2]贵州省高等学校药食两用资源特征风味感知及品质调控工程研究中心,贵州贵阳550005

出　　处：《食品工业科技》2024年第19期17-24,共8页Science and Technology of Food Industry

基　　金：贵州省教育厅青年科技人才成长项目(黔教合KY字[2022]007号);大学生创新训练项目(S202110976022,S202110976006);贵州省高等学校药食两用资源特征风味感知及品质调控工程研究中心(黔教技[2023]042号)。

摘　　要：本文旨在优化八月瓜果皮多糖的提取工艺,评估其体外抗氧化和降糖活性。通过单因素实验结合正交试验优化八月瓜果皮多糖的超声提取工艺;以DPPH自由基清除率及总还原能力为指标测定果皮多糖的抗氧化活性,以α-葡萄糖苷酶和α-淀粉酶抑制率为指标测定其降糖活性。结果显示,八月瓜果皮多糖超声提取的最佳工艺条件为:超声功率280 W、超声时间60 min、提取温度50℃、料液比1:40 g/mL,在此条件下八月瓜果皮多糖的得率为5.91%±0.20%;在测定浓度范围内,果皮多糖具有较好的DPPH自由基清除力和还原能力,其中果皮多糖和VC清除DPPH自由基的半数抑制浓度(IC_(50))分别为0.329、0.077 mg/mL;果皮多糖抑制α-葡萄糖苷酶和α-淀粉酶活性的IC_(50)值分别为0.386、0.307 mg/mL。本研究表明,该工艺条件下纯化的八月瓜果皮多糖具有较好的体外抗氧化性和降糖活性。The aim of this research was to optimize the polysaccharides extraction process from the peels of Akebia trifoliate,and evaluate its in vitro antioxidant and hypoglycemic activity.The ultrasonic-assisted extraction process of polysaccharides from the peels of Akebia trifoliate was optimized using single-factor combined with orthogonal experiment.The antioxidant activity of polysaccharides from the peels of Akebia trifoliate was investigated by using DPPH radical scavenging rate and total reducing ability as the indicators.The hypoglycemic activity of polysaccharides from the peels of Akebia trifoliate was explored usingα-glucosidase andα-amylase inhibition rate as the indicators.The results demonstrated that the optimal ultrasonic-assisted extraction process of polysaccharides from the peels of Akebia trifoliate was ultrasound power 280 W,ultrasound time 60 min,extraction temperature 50℃,solid-liquid ratio 1:40 g/mL,respectively.Under the optimal process condition,the extraction rate of polysaccharides from the peels of Akebia trifoliate was 5.91%±0.20%.Within the measured concentration range,polysaccharides from the peels of Akebia trifoliate had the preferable DPPH radical scavenging and reducing ability.In addition,the IC_(50)values of polysaccharides from the peels of Akebia trifoliate and VC for scavenging DPPH free radicals were 0.329 and 0.077 mg/mL,separately.The IC_(50)values of polysaccharides from the peels of Akebia trifoliate for inhibitingα-glucosidase andα-amylase activity were 0.386 and 0.307 mg/mL,respectively.This research proved that the polysaccharides from the peels of Akebia trifoliate extracted by this optimal process had better in vitro antioxidant and hypoglycemic activity.

关 键 词：八月瓜果皮 多糖 提取工艺 抗氧化 降血糖 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]