二维液相色谱分离鉴定桑葚中多酚类化合物  

Separation and identification of polyphenols in mulberry by two-dimensional liquid chromatography

作　　者：贺增洋 佘世科[1] 徐志强[1] 彭晓萌 孙丽莉 吴攀 许春平 HE Zeng-Yang;SHE Shi-Ke;XU Zhi-Qiang;PENG Xiao-Meng;SUN Li-Li;WU Pan;XU Chun-Ping(Technology Center of China Tobacco Anhui Industrial Co.,Ltd.,Hefei 230088,China;College of Tobacco Science and Technology,Zhengzhou University of Light Industry,Zhengzhou 450002,China)

机构地区：[1]安徽中烟工业有限责任公司技术中心,合肥230088 [2]郑州轻工业大学烟草科学与工程学院,郑州450002

出　　处：《食品安全质量检测学报》2024年第16期292-299,共8页Journal of Food Safety and Quality

基　　金：安徽中烟工业有限责任公司科技项目(2023340000340036);中国烟草总公司重点研发项目(110202102024)。

摘　　要：目的建立一套亲水作用色谱×反相色谱二维液相色谱系统对桑葚中多酚类化合物进行鉴定。方法首先采用单因素实验对色谱系统中一维色谱的流动相A的组成、二维色谱的色谱运行条件以及两维系统接口处的样品环的体积进行优化,然后利用优化系统对桑葚中多酚类化合物进行分离鉴定。结果当一维色谱流动相A中乙酸浓度为2.0%、二维色谱流动相流速为3.3 mL/min、二维色谱分析时间为1.3 min、接口处的样品环体积为150μL时,桑葚提取物中多酚类化合物分离效果最好;使用优化后的系统对桑葚提取物中多酚类化合物进行分离鉴定,成功分离鉴定了桑葚提取物中5种酚类化合物。结论本研究的优化方法可行,优化后的系统可以高效、准确分离桑葚提取物中多酚类化合物,为分离鉴定桑葚中多酚类化合物提供了一定的实践基础。Objective To establish a hydrophilic interaction chromatography×reverse phase chromatography two-dimensional liquid chromatography system for the identification of phenolic compounds in mulberry.Methods The method first used a single factor experiment to optimize the composition of mobile phase A in one-dimensional chromatography,chromatographic operating conditions in two-dimensional chromatography,and the volume of the sample ring at the interface of the two-dimensional system in the chromatographic system.Then,the optimization system was used to separate and identify phenolic compounds in mulberry.Results When the concentration of acetic acid in one-dimensional chromatography mobile phase A was 2.0%,the flow rate of two-dimensional chromatography mobile phase was 3.3 mL/min,the analysis time of two-dimensional chromatography was 1.3 min,and the sample ring volume at the interface was 150μL,the separation effect of phenolic compounds in mulberry extract was the best;the optimized system was used to isolate and identify phenolic compounds in mulberry extract,and 5 kinds of phenolic compounds were successfully isolated and identified in mulberry extract.Conclusion The optimization method of this study is feasible,and the optimized system can efficiently and accurately separate polyphenolic compounds in mulberry extract,providing a certain practical basis for the separation and identification of polyphenolic compounds in mulberry.

关 键 词：桑葚 多酚类化合物 全二维液相色谱法 亲水作用色谱-反相色谱法 

分 类 号：O657.72[理学—分析化学] TS255.7[理学—化学]