不同极性段滇黄精抗氧化及细胞保护作用评价  

The Antioxidant Activities and Cell Protective Effects of Different Polar Samples of Polygonatum kingianum

作　　者：陈凯燕 方语文 张猛[1] 程桂广[1] 刘亚平 周文兵 Chen Kaiyan;Fang Yuwen;Zhang Meng;Cheng Guiguang;Liu Yaping;Zhou Wenbing(Faculty of Food Science and Engineering,Kunming University of Science and Technology,Kunming 650500;Engineering Research Center for Biological Control of Tobacco Diseases and Insect Pests,Yuxi 653100,Yunnan)

机构地区：[1]昆明理工大学食品科学与工程学院,昆明650500 [2]烟草行业病虫害生物防治工程研究中心,云南玉溪653100

出　　处：《中国食品学报》2024年第8期133-142,共10页Journal of Chinese Institute Of Food Science and Technology

基　　金：云南省重大科技计划项目(202202AG050009);云南省应用基础研究面上项目(202101AT070436)。

摘　　要：目的:以食药同源明星植物滇黄精为原料,探讨不同极性段滇黄精的体外抗氧化活性和细胞保护作用。方法:通过D101大孔树脂,以滇黄精根茎甲醇提取物上柱,使用不同体积分数的甲醇水(20%,40%,60%,80%,100%)依次洗脱获得不同极性段的滇黄精样品,测定其总酚、总黄酮含量,通过1,1-二苯基-2-三硝基苯肼(DPPH)和2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)方法评价各极性段滇黄精样品的体外抗氧化活性,利用H2O2诱导的HePG2细胞氧化应激模型测定了细胞内活性氧(ROS)、脂质过氧化物(MDA)的含量和抗氧化酶[超氧化物歧化酶(SOD)、胱甘肽过氧化物酶(GSH-px)]活性,评估不同极性段滇黄精样品的抗氧化活性和细胞保护作用。通过主成分分析(PCA),评估不同极性段滇黄精样品的总酚总黄酮含量、体外抗氧化活性、胞内ROS与MDA含量及胞内抗氧化酶(SOD、GSH-px)活性之间的相关性。结果表明:各极性段样品的抗氧化能力与总酚、总黄酮的含量呈正相关关系,含量越高,抗氧化能力越强。40%和60%甲醇段样品总酚含量(TPC)分别为(40.27±0.59)mg GAE/g提取物和(51.34±2.08)mg GAE/g提取物;总黄酮含量(TFC)分别为(23.95±1.73)mg RE/g提取物和(24.97±2.75)mg RE/g提取物。40%和60%甲醇段样品体外清除自由基的效果显著,能显著抑制细胞内ROS的生成,提高细胞内SOD、GSH-px的水平,降低MDA的含量,体现出较好的抗氧化活性和细胞保护作用。结论:不同极性段滇黄精样品均具有一定的抗氧化活性和细胞保护作用,其中40%和60%甲醇段样品效果最为显著。Polygonatum kingianum,belong to the genus Polygonatum(Liliaceae),mainly distributed in subtropical in the southwest of China,including Guizhou,Sichuan,and Yunnan.The dried rhizomes of P.kingianum have been used as a famous homologous plant of medicine and food for thousands of years.The paper was investigated the antioxidant activities and cell protective effects of Polygonatum kingianum.Using D101 macroporous resin,the methanol extract was eluted by different volume fraction of methanol water(20%,40%,60%,80%,100%)to obtain the different polar samples.The contents of total phenolic(TPC)and total flavonoids(TFC)in different polar samples were determined.The antioxidant activities were evaluated by 2,2-diphenyl-1-picryhydrazyl(DPPH)and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)methods.Furthermore,the cytoprotective effects of different polar samples were performed on hydrogen peroxide(H2O2)-induced oxidative stress in HepG2 cells.The intracellular ROS production,the content of lipid peroxides(MDA)and the activity of enzymes(superoxide dismutase(SOD),glutathione peroxidase(GSH-px))were measured.Principal component analysis(PCA)was used to analyze the correlations among the TPC and TFC,antioxidant activities(DPPH and ABTS),the contents of intracellular ROS and MDA,and the activities of intracellular oxidase(SOD,GSH-px).The results showed that the antioxidant capacities of different polar samples were positively correlated with TPC and TFC.The antioxidant capacity of the sample increased with the increasing of total phenolic and flavonoids content.The TPC of the 40%and 60%methanol samples were(40.27±0.59)mg GAE/g extract and(51.34±2.08)mg GAE/g extract,respectively.While TFC were(23.95±1.73)mg RE/g extract and(24.97±2.75)mg RE/g extract,respectively.The 40%and 60%methanol samples exhibited the highest phenolic and flavonoid contents,and displayed significantly free radical scavenging activities in vitro.Additionally,these samples markedly inhibited intracellular ROS production,enhanced SOD and

关 键 词：滇黄精 不同的极性段 抗氧化 细胞保护 

分 类 号：TS201.4[轻工技术与工程—食品科学]