沙葱多酚提取物抗氧化活性及其对消化酶的抑制作用  

Antioxidant Activities and Inhibitory Effects on Digestive Enzyme of Polyphenolic Extracts from Allium mongolicum Regel

作　　者：卢跃红 王斌 刘偲 祖拜旦·艾买尔 高春燕 Lu Yuehong;Wang bin;Liu Cai;Zupaidano Aimaier;Gao Chunyan(College of Bioscience and Engineering,North Minzu University,Yinchuan 750021)

机构地区：[1]北方民族大学生物科学与工程学院,银川750021

出　　处：《中国食品学报》2024年第8期164-177,共14页Journal of Chinese Institute Of Food Science and Technology

基　　金：青年人才培育项目(2021KYQD37);宁夏自然科学基金项目(2022AAC03263)。

摘　　要：通过体外抗氧化和酶抑制测定体系,评价沙葱游离酚和结合酚提取物的抗氧化能力及其对消化酶的抑制作用。分别采用福林-酚法、NaNO2-AlCl3法和香草醛-盐酸法测定总酚、黄酮和缩合单宁含量;利用DPPH自由基清除活性、铁还原抗氧化能力(FRAP)、Trolox等量抗氧化活性(TEAC)和HO·介导的2-脱氧-D-核糖裂解抑制作用体系,综合评价沙葱多酚提取物的抗氧化能力;采用体外消化酶抑制作用测定方法,评价沙葱多酚提取物对胰脂肪酶和葡萄糖苷酶的抑制作用。结果显示,沙葱多酚提取物总酚、黄酮和缩合单宁的含量范围分别为251.09~521.27μg GAE/mg,148.24~512.94μg CAE/mg和121.33~360.56μg CAE/mg,且不同产地其含量存在差异。沙葱游离酚提取物DPPH自由基清除活性、FRAP和TEAC范围分别为175.02~248.81μmol TE/g、646.22~842.89μmol Fe(II)/g和54.95~87.95μmol TE/g;结合酚提取物DPPH自由基清除活性、FRAP和TEAC范围分别为447.14~623.95μmol TE/g、1051.78~1866.22μmol Fe(II)/g和441.17~714.50μmol TE/g。沙葱游离酚对非定位和定位HO·介导的2-脱氧-D-核糖裂解抑制作用的IC50值范围分别为1.14~2.27 mg/mL和3.39~3.70 mg/mL;沙葱结合酚对非定位和定位HO·介导的2-脱氧-D-核糖裂解抑制作用的IC50值范围分别为0.62~0.94 mg/mL和2.80~3.99 mg/mL。总体而言,沙葱结合酚提取物的抗氧化能力强于游离酚。沙葱游离酚和结合酚提取物对胰脂肪酶和葡萄糖苷酶的抑制作用均属于混合非竞争性抑制,且游离酚提取物的抑制作用强于结合酚。就酚类物质的含量、抗氧化能力和消化酶抑制活性而言,沙葱结合酚提取物的综合品质高于游离酚。The antioxidant capacities and digestive enzyme inhibitory effects of free and bound phenolic extracts from Allium mongolicum Regel were studied through antioxidant and enzyme inhibition assays in vitro.The methods of Folin-Ciocalteu,NaNO2-AlCl3 and vanillin-hydrochloric acid were used for the determination of total phenolics,flavonoids and condensed tannins,respectively.DPPH radical scavenging activity,ferric reducing antioxidant power(FRAP),trolox equivalent antioxidant capacity(TEAC)and inhibitory effects on HO·mediated 2-deoxyribose degradation assays were used to comprehensively evaluate the antioxidant capacity.In vitro digestive enzyme inhibitory test assay was used to evaluate the inhibitory effects of polyphenol extracts from A.mongolicum Regel onα-glucosidase and pancreatic lipase.The content of total phenol,flavone and condensed tannin of polyphenol extracts from A.mongolicum Regel were in the range of 251.09-521.27μg GAE/mg,148.24-512.94μg CAE/mg and 121.33-360.56μg CAE/mg,respectively,and their contents varied from different origins.The DPPH radical scavenging activity,FRAP and TEAC of free phenolic extracts from A.mongolicum Regel were in the range of 175.02-248.81μmol TE/g,646.22-842.89μmol Fe(II)/g and 54.95-87.95μmol TE/g,respectively.The DPPH radical scavenging activity,FRAP and TEAC of bound phenolic extracts from A.mongolicum Regel were in the range of 447.14-623.95μmol TE/g,1051.78-1866.22μmol Fe(II)/g and 441.17-714.50μmol TE/g,respectively.The IC50 values of free phenolic extracts for the inhibition of non-site and site hydroxyl radical-mediated 2-deoxyribose degradation ranged from 1.14 to 2.27 mg/mL and 3.39 to 3.70 mg/mL respectively.The IC50 values of bound phenolic extracts for the inhibition of non-site and site hydroxyl radical-mediated 2-deoxyribose degradation ranged from 0.62 to 0.94 mg/mL and 2.80 to 3.99 mg/mL,respectively.In general,the antioxidant capacity of the bound phenolic extract was stronger than that of free phenolics.The free and bound phenolic extracts inh

关 键 词：沙葱 多酚 抗氧化 酶抑制作用 

分 类 号：TS201.2[轻工技术与工程—食品科学]