介质阻挡等离子体降解乙腈溶液黄曲霉毒素B1规律解析  

Degradation Mechanism of Aflatoxin B1 in Acetonitrile Solvent by DielectricBarrier Discharge Plasma

作　　者：苏迎利 马挺军[1] 魏朝俊 Su Yingli;Ma Tingjun;Wei Chaojun(College of Food Science and Engineering,Beijing University of Agricultural,Beijing 102206;Key Laboratory of Urban Agriculture(North),Ministry of Agriculture and Rural Affairs,Beijing 102206;College of Bioscience and Resources Environment,Beijing University of Agriculture,Beijing 102206)

机构地区：[1]北京农学院食品科学与工程学院,北京102206 [2]农业农村部都市农业(北方)重点实验室,北京102206 [3]北京农学院生物与资源环境学院,北京102206

出　　处：《中国食品学报》2024年第8期473-485,共13页Journal of Chinese Institute Of Food Science and Technology

基　　金：北京市景观休闲农业创新团队项目(BAIC09-2023)。

摘　　要：目的:花生、玉米及坚果的黄曲霉毒素B1污染是影响食品质量安全的关键因素,通过等离子体降解黄曲霉毒素B1来降低污染,并探究降解机理是当前的急迫需求。方法:利用等离子体处理技术,在放电功率200 W,放电时间5 s,放电间距0.4 cm条件下降解乙腈溶液中的黄曲霉毒素B1,采用高效液相色谱-三重四级杆质谱联用仪和高分辨质谱进行分析,以期获得介质阻挡等离子体法降解黄曲霉毒素B1的产物和路径。结果:分析出5种降解产物的分子式及结构式,其降解途径为Criegee机理、苯环甲氧基反应和亲核反应机理。在降解率为35.8%的情况下,根据5种降解产物毒性位点损失的情况,证明介质阻挡等离子体处理之后毒性与降解率一致。结论:本研究为利用介质阻挡等离子体降解花生、玉米和坚果制品中的黄曲霉毒素B1提供理论参考,后续试验可探讨不同降解率下的产物及反应过程,可通过动物实验或细胞实验进一步探索介质阻挡等离子体降解黄曲霉毒素B1后的潜在毒性验证。Objective:Aflatoxin B1 pollution is the key factor affecting food quality and safety.How to degrade aflatoxin B1 to reduce pollution and explore the degradation mechanism is the current urgent demand.Methods:Aflatoxin B1 in acetonitrile solution was degraded by plasma treatment with a discharge power of 200 W,a discharge time of 5 s and a discharge distance of 0.4 cm,and analysed by high performance liquid chromatography-mass spectrometry(HPLC-MS/MS)and high resolution mass spectrometry(HRMS)with the aim of obtaining the products and pathways for the degradation of aflatoxin B1 in the dielectric barrier discharge plasma method.Results:The molecular and structural formulae of five degradation products were analysed,and their degradation pathways were Criegee mechanism,Benzyl Methoxy Reaction and Nucleophilic Reaction.With the degradation rate of 35.8%,it was demonstrated that the toxicity was consistent with the degradation rate after dielectric barrier discharge plasma treatment based on the loss of toxicity sites of the five degradation products.Conclusion:This study provides a theoretical reference for the degradation of aflatoxin B1 in peanut,corn and nut products using dielectric barrier discharge plasma,and the potential toxicity validation after degradation of aflatoxin B1 by dielectric barrier discharge plasma can be further explored in subsequent experiments through animal experiments or cell experiments.

关 键 词：黄曲霉毒素B1 介质阻挡等离子体 高效液相色谱-三重四级杆质谱联用 降解产物 

分 类 号：TS207.5[轻工技术与工程—食品科学] O657.63[轻工技术与工程—食品科学与工程]