Huoxue Jiedu Huayu recipe(活血解毒化瘀方)inhibits macrophage-secreted vascular endothelial growth factor-a on angiogenesis and alleviates renal fibrosis in the contralateral kidneys of unilateral ureteral obstruction rats  

作　　者：GAO Xiaomeng QIANG Panpan CHANG Jingyue FAN Lili YANG Fan XU Qingyou 

机构地区：[1]Graduate School,Hebei University of Chinese Medicine,Hebei Key Laboratory of Integrative Medicine on Liver-Kidney Patterns,Hebei University of Chinese Medicine,Shijiazhuang 050091,China [2]Graduate School,Hebei University of Chinese Medicine,Hebei Key Laboratory of Integrative Medicine on Liver-Kidney Patterns,Hebei University of Chinese Medicine,Institute of Integrative Medicine,Hebei University of Chinese Medicine,Shijiazhuang 050091,China

出　　处：《Journal of Traditional Chinese Medicine》2024年第3期458-467,共10页中医杂志（英文版）

基　　金：Natural Science Foundation-funded Project:Effect of Macrophage-to-Myofibroblast Transition in Contralateral Kidney of Unilateral Ureteral Obstruction Rats Through the Aldosterone/MR/SGK1 Pathway and Inhibition of Chinese Herbs(No.81873251);Natural Science Foundation-funded Project:Aldosterone Stimulates MR Activation to Induce Lymphangiogenesis in the Contralateral Kidney of UUO Rats and the Protective Effect of Yiqi Jiedu Huayu Herbs(No.82174317);Hebei Provincial Postgraduate Innovative Ability Cultivation Funding Project:Aldosterone Induced Macrophages to Secrete VEGFA to Participate in Renal Angiogenesis and the Protective Effect of Huoxue Jiedu Huayu Recipe(CXZZBS2023140);Construction Program of New Research and Development Platform and Institution,Hebei Province Innovation Ability Promotion Plan under Grant No.20567624H。

摘　　要：OBJECTIVE:To elucidate the mechanism by which Huoxue Jiedu Huayu recipe(活血解毒化瘀方,HJHR)regulates angiogenesis in the contralateral kidney of unilateral ureteral obstruction(UUO)rats and the mechanism by which it reduces of renal fibrosis.METHODS:Male Wistar rats were randomly divided into 4 groups:the sham group,UUO group(180 d of left ureter ligation),UUO plus eplerenone(EPL)group,and UUO plus HJHR group.After 180 d of oral drug administration,blood and contralateral kidneys were collected for analysis.Angiogenesis-and fibrosis-related indexes were detected.RESULTS:HJHR and EPL improved structural damage and renal interstitial fibrosis in the contralateral kidney and reduced the protein expression levels ofα-smooth muscle actin(α-SMA),vimentin and collagen I.Moreover,these treatments could reduce the expression of vascular endothelial growth factor-A(VEGFA)by inhibiting the infiltration of macrophages.Furthermore,HJHR and EPL significantly reduced the expression of CD34 and CD105 by downregulating VEGFA production,which inhibited angiogenesis.Finally,the coexpressions of CD34,CD105 andα-SMA were decreased in the HJHR and EPL groups,indicating that endothelial-to-mesenchymal transition was inhibited.CONCLUSIONS:These findings confirm that HJHR alleviates contralateral renal fibrosis by inhibiting VEGFAinduced angiogenesis,encourage the use of HJHR against renal interstitial fibrosis and provide a theoretical basis for the clinical management of patients with CKD.

关 键 词：epithelial-mesenchymal transition ALDOSTERONE EPLERENONE receptors MINERALOCORTICOID unilateral ureteral obstruction Huoxue Jiedu Huayu recipe 

分 类 号：R285.5[医药卫生—中药学]