基于还原氧化石墨烯-聚苯胺/纳米金复合纳米材料的阻抗型电化学适配体传感器检测啶虫脒  

An Impedimetric Aptamer Sensor Based on Reduced Graphene Oxide-Polyaniline/Gold Nanoparticle Nanocomposite for the Detection of Acetamiprid

作　　者：罗丽红 蒋翠文 劳水兵 覃国新 闫飞燕 梁钟丹 韦宇宁 李慧玲 殷玮琰 周玉玲 牙禹 LUO Lihong;JIANG Cuiwen;LAO Shuibing;QIN Guoxin;YAN Feiyan;LIANG Zhongdan;WEI Yuning;LI Huiling;YIN Weiyan;ZHOU Yuling;YA Yu(Agro-products Quality Safety and Testing Technology Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China;Wuhan Textile University,Wuhan 430200,China;Hainan Institute for Food Control,Haikou 570311,China)

机构地区：[1]广西壮族自治区农业科学院农产品质量安全与检测技术研究所,广西南宁530007 [2]武汉纺织大学,湖北武汉430200 [3]海南省食品检验检测中心,海南海口570311

出　　处：《食品科学》2024年第19期242-250,共9页Food Science

基　　金：广西自然科学基金项目(2023GXNSFAA026442);广西农业科学院基本科研业务专项(桂农科2024YP113;桂农科2023YM117);广西农业科学院稳定资助科研团队项目(桂农科2021YT138);广西农业科学院优势学科团队项目(桂农科2021YT136);科技先锋队“强农富民”“六个一”专项行动(桂农科盟202416);国家市场监管重点实验室(热带果蔬质量与安全)基础应用及重点研究课题(KF-2023001)。

摘　　要：基于纳米金(Aunanoparticles,AuNPs)/还原氧化石墨烯(reduced graphene oxide,rGO)-聚苯胺(polyaniline,PANi)复合材料制备一种高灵敏、高选择性的啶虫脒(acetamiprid,ACE)电化学适配体传感器(6-巯基-1-己醇(6-mercapto-1-hexanol,MCH)/Apt/AuNPs/rGO-PANi)。采用透射电镜、X射线衍射和扫描电镜/能谱等多种技术对所合成的纳米材料及电极的修饰界面进行了表征,确证了形貌及结构。使用电化学技术研究不同电极的电化学性能,发现AuNPs/rGO-PANi复合材料修饰电极具有较大的电化学活性面积和优异的电子传输能力,可为适配体的固定及电化学检测ACE提供一个理想的平台。在最优条件下,MCH/Apt/AuNPs/rGO-PANi对ACE检测的线性范围为1.0~100000.0 ng/L,检出限为0.15 ng/L(信噪比=3)。100倍的常见共存农药分子不会对ACE的检测产生明显干扰,将该适配体传感器用于青瓜和红薯叶样品检测,回收率为94.5%~98.5%,结果的准确性也得到了液相色谱-质谱法的验证。In this study,a highly sensitive and selective electrochemical aptamer sensor(6-mercapto-1-hexanol(MCH)/Apt/AuNPs/rGO-PANi)for acetamiprid(ACE)was prepared based on Au nanocomposites(AuNPs)/reduced graphene oxide(rGO)-polyaniline(PANi).The morphology and structure of the synthesized nanomaterial and the interface of different electrodes were characterized by transmission electron microscopy(TEM),X-ray diffraction(XRD)and scanning electron microscopy(SEM)/energy dispersive X-ray spectrometry(EDX).The electrochemical properties of different electrodes were studied by cyclic voltammetry and electrochemical impedance spectroscopy,and it was found that the AuNPs/rGOPANi modified electrode showed a large electrochemical active area and excellent electrochemical properties,which could provide an ideal platform for aptamer fixation and electrochemical sensing of ACE.Under optimal conditions,the linear range of MCH/Apt/AuNPs/rGO-PANi for ACE detection was 1.0–100000.0 ng/L,and the detection limit was 0.15 ng/L at a signal-to-noise(RSN)of 3.A 100-fold excess of common co-existing pesticides did not significantly interfere with the detection of ACE.The prepared aptamer sensor was applied to the detection of cucumbers and sweet potato leaves with recoveries ranging from 94.5%to 98.5%.The accuracy of the developed method was verified by liquid chromatography-mass spectrometry(LC-MS).

关 键 词：适配体传感器 啶虫脒 电化学阻抗法 食品分析 

分 类 号：TS207.3[轻工技术与工程—食品科学]