赶黄草叶不同溶剂提取物中活性成分、抗氧化活性及胰脂肪酶抑制活性对比  

Comparison of Active Components,Antioxidant Activity and Pancreatic Lipase Inhibitory Activity in Different Solvent Extracts of Penthorum chinense Leaves

作　　者：刘佳 向卓亚[2] 朱柏雨 夏陈 朱永清[1,2] 杨星 陈松[3] LIU Jia;XIANG Zhuoya;ZHU Boyu;XIA Chen;ZHU Yongqing;YANG Xing;CHEN Song(College of Food and Biological Engineering,Chengdu University,Chengdu 610106,China;Institute of Agro-products Processing Science and Technology(Institute of Food Nutrition and Health),Sichuan Academy of Agricultural Sciences,Chengdu 610066,China;Gucui Biotechnology Co.,Ltd.,Luzhou 646500,China)

机构地区：[1]成都大学食品与生物工程学院,四川成都610106 [2]四川省农业科学院农产品加工所(四川省农业科学院食物与营养健康研究所),四川成都610066 [3]古萃(古蔺)生物科技有限公司,四川泸州646500

出　　处：《食品工业科技》2024年第20期223-231,共9页Science and Technology of Food Industry

基　　金：四川省农业科学院“1+9揭榜挂帅”项目(1+9KJGG007);四川道地中药材创新团队项目(SCCXTD-2020-19)。

摘　　要：赶黄草叶活性成分丰富,本文对赶黄草叶不同溶剂提取(80%丙酮、80%乙醇、80%甲醇、水)物中活性成分含量、体外抗氧化能力以及胰脂肪酶抑制能力进行对比研究。结果显示,80%丙酮提取物中总多酚含量最高(250.04 mg/g),而80%甲醇和80%乙醇提取物中总黄酮(176.96 mg/g和174.89 mg/g)和总原花青素含量(43.05 mg/g和42.87 mg/g)最高。除芦丁、异槲皮苷、山奈酚-3-O-芸香糖苷、乔松素-7-O-葡萄糖苷、乔松素-7-O-(3''-O-没食子酰基-4'',6''-六羟基联苯二甲酰基)-β-葡萄糖苷和赶黄草苷A外,三种有机溶剂提取物中9种多酚类化合物含量无显著性差异,均显著高于水提物(P<0.05)。80%甲醇提取物和80%乙醇提取物对1,1-二苯基-2-三硝基苯肼(DPPH)自由基(IC_(50)=42.17μg/mL和42.21μg/mL,P>0.05)清除能力最强,80%丙酮提取物对2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS^(+))自由基清除能力最强(IC_(50)=48.92μg/mL),铁离子还原能力最强为80%乙醇提取物。此外,80%丙酮对体外胰脂肪酶抑制能力最强(IC_(50)=0.46 mg/mL)。相关性分析表明,赶黄草叶体外DPPH和ABTS~+自由基清除能力以及胰脂肪酶抑制能力与11种多酚类化合物呈显著或极显著正相关(P<0.05,P<0.01)。综上,结合不同溶剂提取物中活性成分含量、体外活性以及溶剂毒性影响,本研究建议将80%乙醇作为赶黄草叶提取溶剂,为赶黄草叶进一步开发提取利用提供理论依据。The Penthorum chinense Pursh leaves were rich in active ingredients.The active ingredient content,in vitro antioxidant capacity and pancreatic lipase inhibition ability of P.chinense leaves extracted by different solvents(80%acetone,80%ethanol,80%methanol,water)were compared in this study.The results showed that the total phenolic content(250.04 mg/g)extracted by 80%acetone was highest,while both the total flavonoid content(176.96 mg/g and 174.89 mg/g)and total proanthocyanidins content(43.05 mg/g and 42.87 mg/g)extracted by 80%methanol and 80%ethanol were highest.Except for rutin,isoquercitrin,kaempferol-3-O-rutinoside,pinocembrin 7-O-D-glucoside,pinocembrin 7-O-(3''-O-galloy-4'',6''-hexahydroxydiphenoyl)-β-glucoside and thonningianin A,the content of nine phenolic compounds showed insignificant differences among three organic solvent extracts,which were significantly higher than those in water extract(P<0.05).Both 80%ethanol extract and 80%methanol extract showed the strongest DPPH free radical scavenging ability(IC50=42.17μg/mL and 42.21μg/mL,P>0.05),while 80%acetone extract showed the strongest ABTS+free radical scavenging ability(IC50=48.92μg/mL)and 80%ethanol extract also showed the strongest ferric reducing antioxidant power.In addition,in vitro pancreatic lipase inhibitory activity of 80%acetone extract(IC50=0.46 mg/mL)was the strongest.Correlation analysis showed that DPPH and ABTS+free radical scavenging ability and pancreatic lipase inhibition ability were significantly or extremely significantly positively correlated with 11 phenolic compounds(P<0.05,P<0.01).Above all,in view of the active ingredient content,in vitro biological activity and solvent toxicity,this study recommend 80%ethanol as the extract solvent of P.chinense leaves,which would provide the theoretical basis for the further development,extraction and utilization of P.chinense leaves.

关 键 词：赶黄草叶 溶剂提取 活性成分 抗氧化活性 胰脂肪酶抑制活性 

分 类 号：TS207.3[轻工技术与工程—食品科学]