肠膜明串珠菌细菌素抗金黄色葡萄球菌的生物学特性分析  

Biological characterization of a bacteriocin produced by Leuconostoc mesenteroides inhibiting Staphylococcus aureus

作　　者：王嘉悦 潘果 钟忻桐 苏惠 路雨 李国江 董文龙 WANG Jiayue;PAN Guo;ZHONG Xintong;SU Hui;LU Yu;LI Guojiang;DONG Wenlong(Animal Science and Technology College,Jilin Agricultural Science and Technology University,Jilin 132109,Jilin,China;Jilin Provincial Key Laboratory of Preventive Veterinary Medicine,Jilin 132109,Jilin,China;Science and Technology Innovation Center of Pig Ecological Breeding and Disease Control,Jilin 132109,Jilin,China)

机构地区：[1]吉林农业科技学院动物科技学院,吉林吉林132109 [2]吉林省预防兽医学重点实验室,吉林吉林132109 [3]吉林省猪生态养殖及疫病防控科技创新中心,吉林吉林132109

出　　处：《微生物学通报》2024年第9期3562-3571,共10页Microbiology China

基　　金：吉林省科技创新平台补助项目(YDZJ202302CXJD038)。

摘　　要：【背景】金黄色葡萄球菌(Staphylococcus aureus)是导致全球耐药负担加重的重要原因,乳酸菌细菌素是治疗致病性细菌感染的一种新型的手段,具有广阔的应用前景。【目的】筛选抑制金黄色葡萄球菌的乳酸菌细菌素。【方法】采用琼脂扩散法筛选产细菌素的乳酸菌;通过16S rRNA基因测序鉴定菌株,并利用MEGA 11构建系统发育树;排除酸和过氧化氢影响后,经生物学特性分析确定菌株细菌素的抗酶解性、pH稳定性和稳定性;测定菌株的生长曲线、产酸能力并观察其生长特性;对菌株进行模拟胃液耐受能力的检测。【结果】从延吉南瓜中分离获得一株肠膜明串珠菌(Leuconostoc mesenteroides)WJY-6,该菌株分泌的细菌素对不同源金黄色葡萄球菌表现出抑菌效果,并且具有良好的抗酶解性,在100℃和121℃处理后抑菌活性仍能维持在35%以上,该细菌素在pH 3.0−5.0条件下抑菌活性最佳;该菌株培养8 h时pH值下降到5.0以下,抑菌效果稳步增加,在18 h进入生长稳定期;该菌株在模拟胃液中有极强的存活能力,并能够大幅增殖。【结论】肠膜明串珠菌WJY-6有着极强的产酸能力,在模拟胃液中耐受能力良好,其所分泌的细菌素具备优异的理化特性,并且对金黄色葡萄球菌存在显著的抑制活性,有望成为防治金黄色葡萄球菌感染相关疾病的新型替代抗生素产品。[Background]Staphylococcus aureus is a key reason for the increase in the global drug resistance burden.Bacteriocins produced by lactic acid bacteria are a new method to treat pathogenic bacterial infections and has broad application prospects.[Objective]To screen the bacteriocins of lactic acid bacteria that inhibit S.aureus.[Methods]The agar diffusion method was used to screen the bacteriocin-producing lactic acid bacteria.Strains were identified by 16S rRNA gene sequencing and a phylogenetic tree was constructed using MEGA 11.After the effects of acids and hydrogen peroxide were excluded,the anti-enzymolysis property,pH stability,and thermostability of the bacteriocin produced by this strain were characterized.The growth curve,acid production capacity,and tolerance to simulated gastric fluid of the strain were studied.[Results]A strain named Leuconostoc mesenteroides WJY-6 was isolated from pumpkin in Yanji.It secreted a bacteriocin showing inhibitory effects on S.aureus from different sources.The bacteriocin exhibited a good anti-enzymolysis property and maintained over 35%of the inhibitory effect after being treated at 100°C and 121°C.The bacteriocin showcased the strongest inhibitory activity at pH 3.0–5.0.The strain entered a stable growth phase after 18 h,with the pH dropping below 5.0 after 8 h,which steadily improved the inhibitory effect.The strain showed strong survival ability and proliferated actively in the simulated gastric fluid.[Conclusion]L.mesenteroides WJY-6 has a strong acid production ability and good tolerance to simulated gastric fluid.Moreover,it secretes a bacteriocin with excellent physical and chemical properties and inhibitory effect on S.aureus.The bacteriocin is expected to become a new alternative of antibiotics for the prevention and treatment of S.aureus.

关 键 词：金黄色葡萄球菌 乳酸菌 肠膜明串珠菌 细菌素 生物学特性 

分 类 号：S859.7[农业科学—临床兽医学]