Trigonelline mitigates bleomycin-induced idiopathic pulmonary fibrosis in mice  

作　　者：Swapnil Gavhane Chandrakant Gawli Sachin Kumar Biswajit Das Gayatri Marathe Vishal S.Patil Harun M.Patel Basavaraj Bommanahalli Chanakya Nath Kundu Chandragouda R.Patil 

机构地区：[1]R.C.Patel Institute of Pharmaceutical Education and Research,Karwand Naka,Shirpur,Maharashtra 425405,India [2]Department of Pharmacology,Delhi Pharmaceutical Sciences and Research University,New Delhi 110017,India [3]School of Biotechnology,Kalinga Institute of Industrial Technology,Bhubaneshwar 751024,India [4]Gadag Institute of Medical Sciences,Gadag,Karnataka 582103,India

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2024年第9期391-400,I0008-I0291,共294页亚太热带生物医学杂志（英文版）

摘　　要：Objective:To evaluate the effect of trigonelline on bleomycin-induced idiopathic pulmonary fibrosis(IPF)and to explore its underlying mechanisms using network pharmacology.Methods:IPF was induced in C57BL/6 mice by a single intratracheal instillation of bleomycin(5 mg/kg).Trigonelline was administered at doses of 25,50,and 100 mg/kg/day orally from the 2nd day post-bleomycin induction up to the 14th day.In IPF-induced mice,lung coefficient,immune cell infiltration in bronchoalveolar lavage fluid,and oxidative stress were measured.Histological alterations in lung tissues were also assessed.Moreover,network pharmacology approach was conducted to reveal molecular interactions of bleomycin and trigonelline with targets of IPF.Results:Trigonelline treatment reduced bleomycin-induced oxidative stress and immune cell infiltration,and mitigated physiological changes in the lung tissues of mice.Moreover,trigonelline alleviated bleomycin-induced histological alterations in lung tissues.Network pharmacology analysis showed that bleomycin and trigonelline interacted with IPF targets,such as NFKB1,HDAC2,HIF1A,and TLR4.Conclusions:The interaction of trigonelline with key IPF targets and its ameliorative effects on lung damage and oxidative stress highlight its potential in treating IPF.It may be considered an antifibrotic agent for further clinical development.

关 键 词：TRIGONELLINE Idiopathic pulmonary fibrosis ANTIFIBROTIC BLEOMYCIN Network pharmacology 

分 类 号：R563[医药卫生—呼吸系统]