Thrombopoietin ameliorates doxorubicin-induced toxicities in H9c2 myocardiocytes by inhibiting oxidative stress through the SIRT1/p38 MAPK signaling pathway  

作　　者：Xu-Han Zuo Yu Huang Bo-Cen Chen Ming-Yue Zhu Cai-Cai Zhang Han-Yi Jiao Li-Fang Lu Man Xiao Han Wang 

机构地区：[1]Key Laboratory of Tropical Translational Medicine of Ministry of Education,School of Basic Medicine and Life Sciences,Hainan Medical University,Haikou,Hainan,China [2]Experiment and Teaching Center for Clinical Skills,Hainan Medical University,Haikou,Hainan,China

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2024年第9期410-416,共7页亚太热带生物医学杂志（英文版）

基　　金：supported by the Natural Science Foundation of Hainan Province High-level Talent Project(grant number 820RC644);Innovative Research Projects for Postgraduate Students at Hainan Medical University(grant number HYYS2022B08).

摘　　要：Objective:To explore whether thrombopoietin can exert a protective effect against doxorubicin-induced cardiotoxicity by modulating the sirtuin 1(SIRT1)signaling pathway.Methods:H9c2 cell viability was determined by CCK-8 and cardiomyocyte apoptosis was detected by TUNEL assay.The protein expressions of SIRT1 and p38 MAPK were measured by Western blot.RT-qPCR was also used to determine SIRT1 mRNA expression.In addition,intracellular reactive oxygen species levels and antioxidant enzyme activities were evaluated.Results:Thrombopoietin treatment reversed doxorubicin-induced decline in H9c2 cell viability.It also increased SIRT1 and decreased p-p38 MAPK protein expressions.In addition,thrombopoietin significantly attenuated doxorubicin-induced apoptosis and oxidative stress,and enhanced antioxidant enzyme activities.However,silencing SIRT1 abrogated the protective effects of thrombopoietin,as evidenced by reduced cell viability and increased oxidative stress and reactive oxygen species levels.Conclusions:Thrombopoietin alleviates doxorubicin-induced cardiomyocyte injury by reducing oxidative stress and apoptosis via the SIRT1/p38 MAPK pathway.However,its protective effects need to be further verified in animal tests.

关 键 词：DOXORUBICIN THROMBOPOIETIN Oxidative stress Sirtuin 1 CARDIOTOXICITY 

分 类 号：R542.2[医药卫生—心血管疾病]