蓝靛果花青素抗氧化作用及机制  

Antioxidant Activity and Mechanism of Anthocyanins from Lonicera caerulea

作　　者：曹柏营[1] 杨海蛟 侯宇[3] 王家利 乔新宇 陈英伟 刘馨阳 CAO Baiying;YANG Haijiao;HOU Yu;WANG Jiali;QIAO Xinyu;CHEN Yingwei;LIU Xinyang(School of Biological and Food Engineering,Jilin Engineering Normal University,Changchun 130052,Jilin,China;Dunhuang City Market Supervision and Administration Bureau,Dunhuang 736200,Gansu,China;Jilin Province Product Quality Supervision and Inspection Institute,Changchun 130103,Jilin,China)

机构地区：[1]吉林工程技术师范学院生物与食品工程学院,吉林长春130052 [2]敦煌市市场监督管理局,甘肃敦煌736200 [3]吉林省产品质量监督检验院,吉林长春130103

出　　处：《食品研究与开发》2024年第19期53-59,共7页Food Research and Development

基　　金：吉林省科技发展计划项目(20200702052NC)。

摘　　要：以蓝靛果花青素为原材料,评价蓝靛果花青素的体外抗氧化作用,从Nrf2-Keap1-ARE通路关键因子的调控角度探讨蓝靛果花青素的抗氧化机制。结果表明,蓝靛果花青素对DPPH自由基、ABTS+自由基和羟自由基的清除率分别为92.48%、99.82%、95.67%,总抗氧化能力为186.62 U/mg,蓝靛果花青素可提高H2O2诱导Caco-2细胞损伤的超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)和谷胱甘肽过氧化物酶(glu-tathione per-oxidase,GSH-Px)活性,降低丙二醛(malonyldialdehyde,MDA)水平,上调Nrf2-Keap1-ARE通路中的核因子E2相关因子2(nuclear factor erythroid 2-related factor 2,Nrf2)mRNA表达量,上调血红素加氧酶l(heme oxygenase,HO-1)和醌羟基氧化还原酶l(quinone oxidoreductase 1,NQO1)mRNA表达量,下调Kelch样ECH关联蛋白1(recombinant kelch Like ECH associated protein 1,Keap1)mRNA表达量,激活细胞抗氧化酶的活性,发挥氧化损伤的保护作用,抗氧化作用与维生素C(vitamin,VC)和特丁基对苯二酚(tert-butylhydroquinone,t-BHQ)相当。Using Lonicera caerulea anthocyanins as raw materials,this study was evaluated the in vitro antioxi-dant activity and mechanism of anthocyanins from the perspective of key regulatory factors in the Nrf2-Keap1-ARE pathway.The results showed that the scavenging rates of L.caerulea anthocyanins for DPPH radicals,ABTS+radicals,and hydroxyl radicals were 92.48%,99.82%,and 95.67%,respectively.The total antioxi-dant capacity was 186.62 U/mg.L.caerulea anthocyanins could increase the levels of superoxide dismutase(SOD),catalase(CAT),and glutathione peroxidase(GSH-Px)in H2O2-induced Caco-2 cell damage,reduce the level of malondialdehyde(MDA),upregulate the mRNA expression of nuclear factor erythroid 2-related factor 2(Nrf2)in the Nrf2-Keap1-ARE pathway,as well as the mRNA expression of heme oxygenase 1(HO-1)and quinone oxidoreductase 1(NQO1),and downregulate the mRNA expression of Kelch-like ECH-associ-ated protein 1(Keap1).L.caerulea anthocyanins activated cellular antioxidant enzymes and exerted protective effects against oxidative damage,with antioxidant effects comparable to those of vitamin C(VC)and tert-butyl-hydroquinone(t-BHQ).

关 键 词：蓝靛果 花青素 抗氧化活性 氧化应激 Nrf2-Keap1-ARE信号通路 

分 类 号：TS264.4[轻工技术与工程—发酵工程]