小米糠多酚体外降血脂活性的试验研究  

Experimental Study on Hypolipidemic Activity of Millet Bran Polyphenols in Vitro

作　　者：温柔 鲁航 解江琳 孙雅男 李书国[1] WEN Rou;LU Hang;XIE Jianglin;SUN Yanan;LI Shuguo(College of Food and Biology,Hebei University of Science and Technology,Shijiazhuang 050018,China)

机构地区：[1]河北科技大学食品与生物学院,河北石家庄050018

出　　处：《食品科技》2024年第8期247-254,共8页Food Science and Technology

基　　金：河北省重点研发计划项目(21327111D)。

摘　　要：多酚是小米糠中的重要功能活性物质,具有抗氧化、抗菌、抗炎、抑制α-糖苷酶、二肽基肽酶IV、调节肠道菌群等诸多生物活性。文章研究了小米糠游离酚、结合酚对胆酸盐的结合能力以及对胰脂肪酶、胆固醇酯酶的抑制作用;通过体外胆酸盐结合能力测定实验,表明小米糠游离酚的胆酸盐结合能力优于结合酚;通过酶促动力学模型研究小米多酚类物质对脂肪代谢关键酶胰脂肪酶、胆固醇酯酶的抑制作用。结果表明:游离酚、结合酚对胰脂肪酶的抑制率分别为51.8%、56.2%,其半抑制浓度IC_(50)值分别为0.93、0.78 mg/mL,通过酶促动力学模型分析其抑制类型分别为可逆竞争性、可逆非竞争性抑制。小米糠游离酚、结合酚对胆固醇酯酶的抑制率分别为64.8%、55.6%,其半抑制浓度IC_(50)值分别为81.27、232.38μg/mL,通过动力学模型分析,二者的抑制类型均为可逆非竞争性,因此小米糠多酚可应用于调节血糖和调节血脂功能食品的开发。Polyphenols are important functional active substances in millet bran,which have the effects of antioxidant,antibacterial,anti-inflammatory and inhibition ofα-glycosidase,dipeptidyl peptidase IV activity,and regulation of gut microbiota.In this article,the binding ability of free polyphenol and conjugated polyphenol in millet bran to cholate and the inhibitory effect of pancreatic lipase and cholesterol esterase were investigated.The cholate binding ability assessment results showed that the cholate-binding ability of millet bran free phenol was better than that of conjugated phenol in vitro.The inhibitory effect of millet polyphenols on pancreatic lipase and cholesterol esterase as key enzymes in fat metabolism was studied by enzymatic kinetic model.The results showed that the inhibition rates of free polyphenol and conjugated polyphenol on pancreatic lipase were 51.8%and 56.2%,respectively,and their IC_(50)values were 0.93 mg/mL and 0.78 mg/mL,respectively,and the inhibition types were reversible competitive and reversible non-competitive inhibition by enzymatic kinetic model.The inhibition rates of free polyphenol and conjugated polyphenol on cholesterol esterase were 64.8%and 55.6%,respectively,and the IC_(50)values were 81.27μg/mL and 232.38μg/mL,respectively,and the inhibition types were reversible non-competitive and reversible non-competitive inhibition types,respectively.Therefore,millet bran polyphenols can be applied to the development of functional foods that regulate blood glucose and blood lipid.

关 键 词：小米糠多酚 降血脂活性 胰脂肪酶 胆固醇酯酶 酶活抑制动力学 

分 类 号：TS201.2[轻工技术与工程—食品科学]