黑曲霉转化橙皮苷制备橙皮素的条件优化及其抗氧化活性的比较  

Optimization of the Conditions for Converting Hesperidin to Hesperetin by Aspergillus niger and Comparison of Their Antioxidant Activities

作　　者：陈玉婷[1,2,3] 刘昊澄 徐玉娟 吴继军[1] 余元善[1] 温靖[1] 傅曼琴[1,2] CHEN Yuting;LIU Haocheng;XU Yujuan;WU Jijun;YU Yuanshan;WEN Jing;FU Manqin(Sericultural&Agri-Food Research Institute Guangdong Academy of Agricultural Sciences,Key Laboratory of Functional Foods,Ministry of Agriculture,Guangdong Key Laboratory of Agricultural Products Processing,Guangzhou 510610,China;Heyuan Branch,Guangdong Laboratory for Lingnan Modern Agriculture,Heyuan 517000,China;College of Food and Pharmaceutical Engineering,Wuzhou University,Wuzhou 543002,China)

机构地区：[1]广东省农业科学院蚕业与农产品加工研究所,农业部功能食品重点实验室,广东省农产品加工重点实验室,广东广州510610 [2]岭南现代农业科学与技术广东省实验室河源分中心,广东河源517000 [3]梧州学院食品与制药工程学院,广西梧州543002

出　　处：《现代食品科技》2024年第9期65-73,共9页Modern Food Science and Technology

基　　金：岭南现代农业科学与技术广东省实验室河源分中心自主科研项目(DT20220028);广州市科技计划项目(2023B03J1370);国家重点研发计划项目(2021YFD1600104);广东省现代农业产业技术体系创新团队建设项目(2023KJ110);广东省农业科学院项目(R2020PY-JX011,202109TD);。

摘　　要：该研究采用微生物转化法,以橙皮苷为底物,利用黑曲霉菌株(Aspergillus niger CP-2)进行定向生物转化制备橙皮素,通过对黑曲霉菌种接种量、pH值、底物浓度、反应温度以及恒温摇床转速进行单因素实验,并选择接种量、pH值、底物浓度三个重要影响因素进行正交实验,以探究出最适转化条件。结果表明:通过高效液相色谱法(HPLC)对Aspergillus niger CP-2生物转化的产物鉴定,确定为橙皮素,最终确定的最佳工艺为:黑曲霉接种量为10%、pH值为7.5、橙皮苷底物浓度为1.0 mmol/L、反应温度为37℃、恒温摇床转速为200 r/min。在此反应体系下反应24 h后,得到的橙皮素产率为63.90%;此外,转化产物橙皮素的抗氧化活性以及对H_(2)O_(2)损伤HCK-8细胞的保护能力均高于转化底物橙皮苷,因此,黑曲霉的生物转化技术可为柑橘属副产物活性物质的高值化利用提供理论依据。In this study,microbial transformation method was used to prepare hesperetin by directed biotransformation using Aspergillus nigerstrain(A.niger CP-2)with hesperidin as the substrate.Single factor experiments were carried out on the inoculation amount of A.niger strain,pH,substrate concentration,reaction temperature,and speed of the constant-temperature shaker,then the three important influencing factors,inoculation amount,pH,and substrate concentration were selected for the Orthogonal experiments to investigate the optimal transformation conditions.The results showed that the product of A.niger CP-2 biotransformation was identified as hesperetin by high performance liquid chromatography(HPLC),and the optimal process was determined as follows:inoculum amount of A.niger was 10%,pH value was 7.5,the concentration of hesperidin substrate was 1.0 mmol/L,the temperature of the reaction was 37℃,and the speed of the shaking machine was 200 r/min.After 24 h of reaction in this reaction system,the yield of hesperetin was 63.90%.Moreover,the antioxidant activity of the transformed product,hesperetin,and its protective ability against H_(2)O_(2)-induced damage to HCK-8 cells were higher than those of the transformation substrate,hesperidin.Therefore,the biotransformation technology using A.niger can provide a theoretical basis for the high-value utilisation of the active substances in the citrus by-products of the genus Citrus.

关 键 词：黑曲霉 橙皮素 橙皮苷 抗氧化活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]