酱香型白酒寡肽抗Hp感染胃黏膜上皮细胞炎性损伤的作用  

The Effect of Oligopeptides from Soy Sauce-Type Baijiu on Helicobacter pylori-Induced Inflammatory Damage in Gastric Epithelial Cells

作　　者：关祉成 马燕飞 林沛纯 曾传玲 刘杰 刘晓宇[1] Guan Zhicheng;Ma Yanfei;Lin Peichun;Zeng Chuanling;Liu Jie;Liu Xiaoyu(Institute of Allergic Reactions and Immunology,Shenzhen University,Shenzhen 518060,Guangdong)

机构地区：[1]深圳大学过敏反应与免疫学研究所,广东深圳518060

出　　处：《中国食品学报》2024年第9期122-130,共9页Journal of Chinese Institute Of Food Science and Technology

摘　　要：目的:探讨酱香型白酒中寡肽类成分(JOPs)缓解由幽门螺杆菌(Hp)感染导致的胃黏膜上皮细胞炎性损伤的活性及其分子机制。方法:分离酱香型白酒中JOPs并鉴定其氨基酸序列。以Hp感染人胃黏膜上皮细胞(GES-1)构建炎性损伤模型。用CCK 8法检测JOPs对GES-1细胞活力的影响;ELISA法检测JOP-1对Hp感染的GES-1细胞炎性因子分泌的影响;Western blotting检测JOP-1对Hp感染的GES-1细胞NF-κB信号通路及环加氧酶-2 (COX-2)、一氧化氮合成酶(iiNOS)蛋白表达的影响。结果:寡肽JOP-1(Phe-Trp-His-Gly)能够显著提高GES-1细胞活力,抑制Hp感染导致的GES-1细胞活力降低;JOP-1能够显著抑制Hp感染导致的GES-1细胞炎性因子(TNF-α、IL-6、IL-1β、PGE2及NO)过量分泌;同时,JOP-1能够抑制Hp感染导致的GES-1细胞NF-κB信号通路激活,抑制COX-2、iNOS蛋白过表达。结论:酱香型白酒中寡肽类成分能够缓解Hp感染导致的胃黏膜上皮细胞炎性损伤,可通过调控NF-κB信号通路抑制炎性因子过量分泌和炎症相关合成酶的表达,发挥保护活性。Objective:To investigate the activity of oligopeptide components in Soy Sauce-Type Baijiu(JOPs) to alleviate the inflammatory damage of gastric mucosal epithelial cells caused by Helicobacter pylori(Hp) infection and its molecular mechanism.Methods:The JOPs were isolated and amino acid sequence identified.The cell model of human gastric mucosal epithelial cells(GES-1) under Hp-exposure was established.CCK8 assay method was used to detect the cell viability,and ELISA method was applied to evaluate the inflammatory epithelial cytokines levels.Western blotting was applied to detect the protein expression in NF-κB signal pathway.Results:The JOP-1(Phe-Trp-His-Gly) performed the most protective activity against the Hp-induced GES-1 cell viability decline.JOP-1 significantly inhibited Hp-induced inflammatory epithelial cytokines(TNF-α,IL-1β,IL-6,PGE2 and NO) over-release.At the same time,JOP-1 inhibited the Hp-induced NF-κB signal pathway activation,and attenuated the Hp-induced COX-2 and iNOS proteins overexpression.Conclusion:The oligopeptides in Soy Sauce-Type Baijiu can alleviate the Helicobacter pylori induced inflammatory damages on gastric mucosal epithelial cells by regulating NF-κB signaling pathway and the COX-2,iNOS protein expression.Furthermore,the Hp-exposure induced inflammatory epithelial cytokines over-secretion was also inhibited.

关 键 词：酱香型白酒 活性寡肽 幽门螺杆菌 NF-ΚB 

分 类 号：TS262.33[轻工技术与工程—发酵工程]