不同澄清剂体外吸附葡萄酒中赭曲霉毒素A的效果评价  

Evaluation of different clarifiers for in vitro adsorption of ochratoxin A in wine

作　　者：石淼 高琛瑜 董荣 李飞飞 乔丹 刘明臣 薛锋 魏登攀 张珍珍 SHI Miao;GAO Chenyu;DONG Rong;LI Feifei;QIAO Dan;LIU Mingchen;XUE Feng;WEI Dengpan;ZHANG Zhenzhen(College of Food Science and Pharmacy,Xinjiang Agricultural University,Urumqi 830052,China;Turpan Loulan Chateau Co.Ltd.,Shanshan 838299,China)

机构地区：[1]新疆农业大学食品科学与药学学院,新疆乌鲁木齐830052 [2]吐鲁番楼兰酒庄股份有限公司,新疆鄯善838299

出　　处：《食品与发酵工业》2024年第19期135-141,共7页Food and Fermentation Industries

基　　金：新疆维吾尔自治区重点研发计划项目(2020B01005-3);新疆维吾尔自治区重大科技专项(2022A02002-2)。

摘　　要：以‘赤霞珠’干红葡萄酒为研究对象,分析不同质量浓度壳聚糖、明胶和皂土处理对葡萄酒中赭曲霉毒素A(ochratoxin,OTA)吸附效果及理化品质的影响。采用HPLC对酒样中OTA进行分析,结果表明,对于初始质量浓度为3.3μg/L的OTA,1.0 g/L壳聚糖处理48 h可以达到75.87%的吸附率,OTA吸附率相比同质量浓度下的明胶和皂土分别提高了11.27%和21.02%,且OTA吸附率随着澄清剂质量浓度的增加而增大。而澄清剂在吸附葡萄酒中OTA的同时也会引起多酚类物质的损失,0.6 g/L壳聚糖处理下多酚类物质的损失率最小,引起总酚、总花色苷和单宁的损失率分别为15.78%、13.48%、-11.41%,透光率为78.02%,其次为1.0 g/L壳聚糖处理。以澄清剂对OTA吸附率最高、对多酚类物质影响较小为目的筛选出1.0 g/L壳聚糖作为吸附葡萄酒中OTA的最佳处理,在此处理下,OTA吸附率可达75.87%,透光率为78.22%,可为选择适宜的OTA吸附剂提供技术支撑。The dry red wine‘Cabernet Sauvignon’was used as the research object to analyze the effects of different concentrations of chitosan,gelatin,and saponin treatments on the adsorption effect of ochratoxin A(OTA)in wine and the quality of wine.High-performance liquid chromatography(HPLC)was used to analyze OTA in wine samples.The results showed that for an initial concentration of 3.3μg/L OTA,a 48-hour treatment with 1 g/L chitosan could achieve a 75.87%adsorption rate.Compared with gelatin and saponin at the same concentration,the adsorption rate of OTA increased by 11.27%and 21.02%with chitosan,respectively.Additionally,the adsorption rate of OTA increased with an increase in the concentration of the clarifying agent.In terms of the effects of different clarifiers on wine quality,the smallest loss of polyphenols occurred in the 0.6 g/L chitosan treatment.This treatment resulted in a 15.78%loss of total phenols,a 13.48%loss of total anthocyanosides,and a-11.41%loss of tannins,with the wine’s transmittance reaching 78.02%.The 1.0 g/L chitosan treatment followed closely with similar effects.Considering the highest adsorption rate of OTA by the clarifier and the minimal influence on wine quality,the optimal treatment for OTA adsorption in wine was determined to be 1.0 g/L chitosan.Under this treatment,the adsorption rate of OTA reached 75.87%and the transmittance rate was 78.22%,providing technical support for the selection of a suitable OTA adsorbent.

关 键 词：澄清剂 HPLC 赭曲霉毒素A 吸附率 

分 类 号：TS262.6[轻工技术与工程—发酵工程]