Microarray analysis of signalling interactions between inflammation and angiogenesis in subchondral bone in temporomandibular joint osteoarthritis  

作　　者：Wenpin Qin Jialu Gao Jianfei Yan Xiaoxiao Han Weicheng Lu Zhangyu Ma Lina Niu Kai Jiao 

机构地区：[1]Department of Stomatology,Tangdu Hospital&State Key Laboratory of Oral and Maxillofacial Reconstruction and Regeneration&School of Stomatology,The Fourth Military Medical University,Xi’an,Shaanxi Province,China [2]State Key Laboratory of Oral and Maxillofacial Reconstruction and Regeneration&National Clinical Research Centre for Oral Diseases&Shaanxi Key Laboratory of Stomatology,School of Stomatology,The Fourth Military Medical University,Xi’an,Shaanxi Province,China

出　　处：《Biomaterials Translational》2024年第2期175-184,共10页生物材料转化电子杂志（英文）

基　　金：supported by the National Key Research and development Programme,No.2023YFC2509100;National Natural Science Foundation of China,No.82170978;Distinguished Young Scientists Funds of Shaanxi Province,No.2021JC-34(all to JK).

摘　　要：Inflammation and angiogenesis,the major pathological changes of osteoarthritis(OA),are closely associated with joint pain;however,pertinent signalling interactions within subchondral bone of osteoarthritic joints and potential contribution to the peripheral origin of OA pain remain to be elucidated.Herein we developed a unilateral anterior crossbite mouse model with osteoarthritic changes in the temporomandibular joint.Microarray-based transcriptome analysis,besides quantitative real-time polymerase chain reaction,was performed to identify differentially expressed genes(DEGs).Overall,182 DEGs(fold change≥2,P<0.05)were identified between the control and unilateral anterior crossbite groups:168 were upregulated and 14 were downregulated.On subjecting significant DEGs to enrichment analyses,inflammation and angiogenesis were identified as the most affected.Inflammation-related DEGs were mainly enriched in T cell activation and differentiation and in the mammalian target of rapamycin/nuclear factor-κB/tumour necrosis factor signalling.Furthermore,angiogenesis-related DEGs were mainly enriched in the Gene Ontology terms angiogenesis regulation and vasculature development and in the KEGG pathways of phosphoinositide 3-kinase-protein kinase B/vascular endothelial growth factor/hypoxia-inducible factor 1 signalling.Protein-protein interaction analysis revealed a close interaction between inflammation-and angiogenesis-related DEGs,suggesting that phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta(Pi3kcd),cathelicidin antimicrobial peptide(Camp),C-X-C motif chemokine receptor 4(Cxcr4),and MYB proto-oncogene transcription factor(Myb)play a central role in their interaction.To summarize,our findings reveal that in subchondral bone of osteoarthritic joints,signal interaction is interrelated between inflammation and angiogenesis and associated with the peripheral origin of OA pain;moreover,our data highlight potential targets for the inhibition of OA pain.

关 键 词：ANGIOGENESIS INFLAMMATION osteoarthritic pain OSTEOARTHRITIS subchondral bone temporomandibular joint(TMJ) 

分 类 号：R684.3[医药卫生—骨科学]