双蛋白天然共生颗粒Pickering纳米乳液的稳定性分析  

Stability Analysis of Pickering Nanoemulsion Covered by Dual-protein Natural Symbiotic Particles

作　　者：邵家起 邓乾春 李康昱[2] 肖军霞 向霞[2] 郑畅[2] 彭登峰[2] SHAO Jiaqi;DENG Qianchun;LI Kangyu;XIAO Junxia;XIANG Xia;ZHENG Chang;PENG Dengfeng(College of Food Science and Engineering,Qingdao Agricultural University,Qingdao 266109,China;Key Laboratory of Oilseeds Processing,Ministry of Agriculture and Rural Affairs,Hubei Engineering Research Center of Oils and Plant Proteins,Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Wuhan 430062,China)

机构地区：[1]青岛农业大学食品科学与工程学院,山东青岛266109 [2]中国农业科学院油料作物研究所,农业农村部油料加工重点实验室,油料脂质化学与营养湖北省重点实验室,湖北武汉430062

出　　处：《食品工业科技》2024年第21期74-84,共11页Science and Technology of Food Industry

基　　金：新疆维吾尔自治区重大科技专项(2022A02004);中国农业科学院“青年英才”引进工程院级人才;“武汉黄鹤英才”产业领军创新类人才项目。

摘　　要：利用双蛋白天然共生颗粒制备Pickering纳米乳液并对其物化稳定性进行分析,以此拓宽其在食品领域中的应用潜力。本研究采用碱提取-等电点沉淀法(Alkali extraction-isoelectric point precipitation,AE)和盐提取-透析法(Salt extraction-dialysis,SE)从大豆-亚麻籽混合粕中富集大豆蛋白(Soy protein)和亚麻籽蛋白(Flaxseed protein),制备双蛋白天然共生颗粒(Natural particles,SFNPs)。首先,利用外观观察、原子力显微镜(Atomic force microscopy,AFM)、圆二色谱和拉曼光谱对两种双蛋白共生颗粒进行表征;其次,将其作为颗粒稳定剂制备Pickering纳米乳液,并对其物理和化学稳定性进行表征。结果表明AE-SFNPs的尺度更为均一,且具有良好的分散性。与SE-SFNPs相比,AE-SFNPs的二级结构中β-折叠占比更高,达51.25%,具有更加致密有序和良好柔性的结构特性。2种双蛋白天然共生颗粒均可制备出Pickering纳米乳液。在28 d的贮藏期间,AE-SFNPs稳定乳液的粒径仅增加了约14 nm,电位基本稳定在-34 mV左右,而SE-SFNPs稳定乳液的粒径增加了约12倍,电位降低了1.32倍。贮藏至第28 d时,AE-SFNPs的稳定性指数(Turbiscan stability index,TSI,5.83),远小于SESFNPs的TSI值(55.13)。尤其是,AE-SFNPs制备的Pickering纳米乳液表现出了比酪蛋白酸钠(食品领域加工常用乳化剂)制备的纳米乳液更佳的物理稳定性和化学稳定性。因此,AE-SFNPs可作为一种天然高效植物蛋白基颗粒稳定剂制备Pickering纳米乳液,为丰富纳米乳液的工业化应用提供了新的解决策略。Pickering nanoemulsions were prepared using dual-protein natural symbiotic particles and their physicochemical stability was analyzed to broaden their potential application in the food industry.This study employed alkali extraction-isoelectric point precipitation(AE)and salt extraction-dialysis(SE)to enrich soy protein and flaxseed protein from soybean-flaxseed mixed meal for fabricating dual-protein symbiotic natural particles(SFNPs).First,two dual-protein symbiotic particles were characterized using appearance observation,AFM,circular dichroism,and Raman spectroscopy.Then,Pickering nanoemulsions were prepared with them as particle stabilizers and characterized for their physical and chemical stability.Results demonstrated that AE-SFNPs had a higher uniformity of size and better dispersibility.AE-SFNPs also showed a higher proportion(as high as 51.25%)ofβ-sheet in its secondary structure,endowing it a more tightly ordered and flexible structure compared with SE-SFNPs.Moreover,both AE-SFNPs and SE-SFNPs could be used to generate Pickering nanoemulsion.During 28 days of storage,the particle size of the stabilized emulsion of AE-SFNPs increased only about 14 nm and itsζ-potential was stabilized at about-34 mV.In contrast,the particle size of the stabilized emulsion of SE-SFNPs increased about 12 fold,and itsζ-potential was decreased 1.32 fold.The stability index(TSI,5.83)of the AE-SFNPs was much smaller than the TSI value(55.13)of the SE-SFNPs when stored until the 28th day.Notably,the Pickering nanoemulsion stabilized by AE-SFNPs exhibited higher physical and chemical stability than that prepared by sodium caseinate(a common emulsifier used in food processing).Therefore,AE-SFNPs can serve as a natural and efficient plant protein-based particle stabilizer to prepare Pickering nanoemulsion.This work provides a new strategy to enhance the industrial application of nanoemulsions.

关 键 词：植物基双蛋白 共生提取 天然颗粒 界面重构 Pickering纳米乳液 

分 类 号：TS201.1[轻工技术与工程—食品科学]