Integrating lipidomics and gut microbiota to study the anti-hyperlipidemia effect of Sargentodoxae Caulis extract  

作　　者：Xiao-Xiao Zhang Li Jia Xiu-Jun Zhan Charity Ngina Qing-Rui Zhang Xiao-Ge Li Qi-Bao Jiang Shi Bai Miao-Miao Jiang 

机构地区：[1]National Key Laboratory of Chinese Medicine Modernization,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China [2]Haihe Laboratory of Modern Chinese Medicine,Tianjin 301617,China [3]School of Medicine,Taizhou University,Taizhou 318000,China.

出　　处：《Traditional Medicine Research》2025年第1期1-11,共11页TMR传统医学研究

基　　金：supported by the Science and Technology Program of Tianjin in China(No.23ZYJDSS00030);the Public Applied Technology Research Project of Zhejiang Province(No.LGD22H090010)。

摘　　要：Background:Sargentodoxae Caulis(SC)is the vine stem of Sargentodoxa Cuneata(Oliv.)Rehd.&E.H.Wilson in C.S.Sargent,and it in traditional Chinese medicine has been known for promoting blood circulation and removing blood stasis as recorded in the ancient book“Illustrated Classics of Materia Medica”.It has been used effectively to treat blood stasis too in modern clinical practice.However,the anti-hyperlipidemia effect of SC is not fully understood.This paper aims at exploring the use of SC stems to improve the balance of blood lipids in the body,and its new role in treating hyperlipidemia.Methods:The effects of SC extract on hyperlipidemia were explored by combining lipidomics and gut microbiota.Secondly,we explored the potential mechanism of SC in treating hyperlipidemia by pathway analysis.Results:The results showed that the stem extract of SC could restore the physiological and biochemical indices of hyperlipidemia in mice,as well as repair the morphological and structural damage to tissues.Compared to the Model group,the SC extract significantly reduced the liver index,epididymal fat index,and Lee’s index.It also significantly decreased serum levels of total cholesterol,triglycerides,low-density lipoprotein cholesterol,peroxisome proliferator-activated receptor gamma(PPAR-γ),D-lactate,and free fatty acids,while significantly increasing the relative content of peroxisome proliferator-activated receptor alpha(PPAR-α).These changes were statistically significant.Non-targeted lipidomics,based on LC-MS,were utilized to investigate the lipid metabolism characteristics in serum,liver,and epididymal fat of the subjects.It was observed that,compared to the blank group,the Model group exhibited significant changes primarily in glycerol lipids and glycerophospholipids.The treatment group also displayed alterations in these lipids.A total of 38,81,and 27 differential lipids were identified in serum,liver,and epididymal fat samples,respectively.Among these,14 common differential lipids were found in both serum and l

关 键 词：HYPERLIPIDEMIA Sargentodoxae Caulis LIPIDOMICS intestinal flora 

分 类 号：R574[医药卫生—消化系统]