啤酒废酵母提取物皮肤美白功效研究  

Skin whitening effect of brewer's spent yeast extract

作　　者：张军 韩文龙 金梦佳 张人平 李乾伟 张云数 刘加乐 熊江珊 王晨 ZHANG Jun;HANWenlong;JINMengjia;ZHANG Renping;LI Qianwei;ZHANG Yunshu;LIU Jiale;XIONG Jiangshan;WANG Chen(College of Food and Chemical Engineering,Zhengzhou University of Technology,Zhengzhou 450044,China)

机构地区：[1]郑州工程技术学院食品与化工学院,河南郑州450044

出　　处：《中国酿造》2024年第10期244-249,共6页China Brewing

基　　金：河南省重点研发与推广专项(科技攻关)项目(222102110313);郑州工程技术学院高层次人才科研项目(500-22077)。

摘　　要：为探究啤酒废酵母(BSY)提取物作为护肤化妆品美白功能因子的可行性。该试验采用乙醇溶液提取得到啤酒废酵母浸膏,并以提取率为评价指标,采用单因素试验和响应面试验优化提取条件。啤酒废酵母浸膏分别用乙醇、石油醚、乙酸乙酯、正丁醇提取得到4种不同极性的提取物,以抗紫外线、抗氧化及酪氨酸酶抑制活性为评价指标,考察4种不同极性提取物对美白效果的影响。结果表明,啤酒废酵母在料液比1∶28(g∶m L)、乙醇体积分数79%、提取温度57℃、提取时间1.5 h的条件下,实际提取率最高为38.1%。不同极性溶剂提取物对紫外线均有吸收效果,石油醚提取物紫外吸收率最高(>87%);乙醇提取物对2,2'-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS)自由基清除率最高,为96.49%;乙酸乙酯提取物对1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除率最高,为93.49%;不同极性溶剂提取物均表现出酪氨酸酶抑制作用,其中石油醚提取物最高,达到33.29%。该研究结果可为啤酒废酵母提取物在护肤化妆品中的应用提供指导,可用于美白护肤产品的研发。In order to explore the feasibility of brewer's spent yeast(BSY)extract as whitening factor of skincare cosmetics.BSY extract was extracted by ethanol solution,and using extraction ratio as evaluation index,the extraction conditions were optimized by single factor tests and response surface tests.The four extracts of different polarity were obtained from BSY extract by ethanol,petroleum ether,ethyl acetate and n-butanol,respectively.Using anti-ultraviolet,anti-oxidation and tyrosinase inhibition activities as evaluation indexes,the effect of four different polar extracts on whitening effect was investigated.The results showed that under the conditions of solid-liquid ratio 1∶28(g∶ml),ethanol volume fraction 79%,extraction temperature and time were 57℃and 1.5 h,the actual extraction yield of BSY was the highest of 38.1%.Different polarities solvents extracts had absorption effects on ultraviolet radiation,and the UV absorption rate of petroleum ether extract was the highest(>87%).The ABTS free radical scavenging rate was the highest in ethanol extract(96.49%).The free radical scavenging rate of DPPH was the highest in ethyl acetate extract(93.49%).Extracts from different polar solvents exhibited tyrosinase inhibition,with petroleum ether extract showed the highest inhibition at 33.29%.The results of this study could provide guidance for the application of BSY extract in skincare cosmetics,and could be used in the development of whitening skincare products.

关 键 词：啤酒废酵母 响应面法 美白 酪氨酸酶活性 

分 类 号：TS261.9[轻工技术与工程—发酵工程]