响应面优化复合酶法提取红薯皮膳食纤维  

Optimization of response surface for extracting dietary fiber from sweet potato peels using complex enzymes

作　　者：林良美 肖少香[2] 张丽红[1] Lin Liangmei;Xiao Shaoxiang;Zhang Lihong(Zhangzhou Institute of Technology,Zhangzhou,Fujian 363000;Hunan Vocational College of Science and Technology,Changsha,Hunan 410004)

机构地区：[1]漳州职业技术学院,福建漳州363000 [2]湖南科技职业学院,湖南长沙410004

出　　处：《粮食科技与经济》2024年第4期96-100,115,共6页Food Science And Technology And Economy

基　　金：漳州职业技术学院校级课题(ZZY2021B141)。

摘　　要：为了消除红薯初加工副产物所带来的环境污染问题,提高红薯产品附加值,以红薯皮为原料,采用复合酶法提取其中的膳食纤维。通过单因素实验探究了复合酶的添加量、酶解时间、酶解pH、酶解温度和料液比对红薯皮膳食纤维得率的影响,并采用Box-Behnken试验优化提取参数。结果表明:红薯皮膳食纤维的最佳提取工艺参数为料液比1∶25(g/mL)、复合酶(纤维素酶∶淀粉酶∶果胶酶质量比=1∶1∶1)添加量0.5%、酶解时间1.8 h、酶解pH 5.5、酶解温度48℃,该工艺条件下红薯皮可溶性膳食纤维得率为23.64%,不溶性膳食纤维得率为39.88%。In order to eliminate the environmental pollution caused by the initial processing by-products of sweet potatoes and improve the added value of sweet potato products,this paper took sweet potato peels as raw materials and explored the effects of compound enzyme addition,enzymatic hydrolysis time,enzymatic hydrolysis pH,enzymatic hydrolysis temperature,and liquid-to-solid ratio on dietary fiber yield through single factor experiments.The response surface method was used to optimize the process parameters.The results showed that the optimal process parameters were a liquid-to-solid ratio of 1:25(g/mL),a compound enzyme addition rate of 0.5%(fiber enzyme:amylase:pectinase=1:1:1),an enzymatic hydrolysis time of 1.8 hours,an enzymatic hydrolysis pH of 5.5,and an enzymatic hydr olysis temperature of 48℃.Under these op-timal process conditions,the yield of soluble dietary fiber from sweet potato peels was 23.64%,and the yield of insoluble dietary fiber was 39.88%.

关 键 词：红薯皮 响应面 复合酶法 膳食纤维 

分 类 号：TS209[轻工技术与工程—食品科学]