玉米及其制品中淀粉糊化度测定方法的研究  

Study on the Determination Method of Starch Gelatinization Degree of Corn and Its Products

作　　者：张幻 ZHANG Huan(Fujian Key Laboratory of Swine Nutrition and Feed/Fujian Aonong Biological Science and Technology Group Inc.,Zhangzhou 363000,China)

机构地区：[1]福建傲农生物科技集团股份有限公司/福建省生猪营养与饲料重点实验室,福建漳州363000

出　　处：《中国猪业》2024年第5期22-32,共11页China Swine Industry

基　　金：福建省种业企业培优项目(2120814-农业生产发展支出)。

摘　　要：本试验旨在建立一种操作简便、精确度高、重复性好的快速测定玉米及其制品淀粉糊化度的测定方法。试验将全糊化试样、待测试样及空白试样与糖化酶溶液混合,水解生成葡萄糖,使用3,5-二硝基水杨酸显色,以比色法测定各溶液吸光度,从而计算淀粉糊化度。本试验通过对影响淀粉糊化度检测结果的关键因素即糖化酶添加量、酶解时间、酶解温度及样品粒度的试验探究,确定了最佳条件:样品过60目分析筛,每0.3 g样品使用1000 U糖化酶,在40~45℃条件下酶解60 min。在此条件下测定玉米、玉米淀粉、膨胀玉米及膨化玉米的淀粉糊化度,结果与行业标准《NY/T4125-2022饲料中淀粉糊化度的测定》进行对比,P值均大于0.05,RSD均小于2%,表明本研究测定淀粉糊化度筛选出的试验条件准确度高、精密度高,并且简便易行,可降低试剂风险及成本,便于检测人员进行质量控制及测定。The purpose of this experiment was to establish a simple,accuracy and reproducible method for starch gelatinization degree of corn and its products.In this experiment,the fully gelatinated sample,the sample to be tested and the blank sample were mixed with the glucoamylase solution,hydrolyzed to glucose.The 3,5-dinitrosalicylic acid was used for color development,and the absorbance of each solution was measured colorimetrically,so as to calculate the starch gelatinization degree.The optimal conditions were determined by investigating the key factors affecting the results of starch pasting degree,such as the amount of glucoamylase added,hydrolysis time,hydrolysis temperature and sample particle size were investigated to determine the best condition,the sample was analyzed by 60 mesh sieve,the samples were digested with 1000 U of saccharase for every 0.3 g of sample for 60 min at 40~45℃.Under these con-ditions,corn,corn starch,expanded corn and puffed corn were compared with the industry standard“NY/T4125-2022 Determination of starch pasting degree in feed”,the P-values were all greater than 0.05,and the RSD were less than 2%,which indicated that the test conditions screened in this study for the determination of starch pasting degree were highly accurate and precise,and were simple and easy to carry out.It could reduce the risk and cost of reagents,and facilitate the quality control and determination by inspectors.

关 键 词：玉米 淀粉糊化度 糖化酶 酶解条件 样品粒度 

分 类 号：S828[农业科学—畜牧学] S816.17[农业科学—畜牧兽医]