红曲霉固态发酵产洛伐他汀工艺条件的研究  

Study on the Process Condition of Lovastatin Production by Monascus Solid-State Fermentation

作　　者：李小娟 涂宗贤 钟梅坤 高全 杨洲 郑春源 高益槐 LI Xiaojuan;TU Zongxian;ZHONG Meikun;GAO Quan;YANG Zhou;ZHENG Chunyuan;GAO Yihuai(Key Laboratory of Natural Bioactive Substances of Fujian Province,Alpha Fujian Bio-technology Co.,Ltd.,Ningde 352000,China)

机构地区：[1]安发(福建)生物科技有限公司,福建省天然生物活性物质企业重点实验室,宁德352000

出　　处：《食品与发酵科技》2024年第5期56-60,共5页Food and Fermentation Science & Technology

摘　　要：该文通过对洛伐他汀生产菌红曲霉S-01固态发酵工艺条件的研究,确定了固态发酵的最佳工艺条件:发酵培养基配方为大米2500 g、甘油75 g、蛋白胨175 g、初始含水量40%,按装载量350 g/1.5 L进行装瓶,接种量25%,于30℃发酵7 d(前发酵),23℃发酵33 d(后发酵),经过优化后的红曲固态发酵产洛伐他汀的含量达到14.38 mg/g,酸式(开环)洛伐他汀含量为9.35 mg/g,酸式(开环)洛伐他汀占比为65.02%。该研究对功能红曲的工业化生产有一定的指导意义。In this paper,the solid-state fermentation conditions of lovastatin producing strain Monascus S-01 were studied,and the best solid-state fermentation conditions were determined:rice 2500 g,glycerium 75 g,peptone 175 g,initial moisture content 40%,bottling with 350 g/1.5 L,inoculum volume 25%,30℃for 7 d(pre-fermentation),23℃for 33 d(post-fermentation),optimized yield of lovastatin by Monascus in solid-state fermentation was 14.38 mg/g,acid(ring-opening)lovastatin was 9.35 mg/g,and the proportion was 65.02%.This study has certain guiding significance for the industrial production of functional Monascus.

关 键 词：红曲霉 固态发酵 洛伐他汀 酸式(开环)洛伐他汀 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]