假单胞菌生物被膜形成机制及控制:基于群体感应与第二信使关系  

Mechanism and Control of Pseudomonas Biofilm Formation:Based on the Relationship between Quorum Sensing and the Second Messenger

作　　者：袁旖旎 周广晖 董鹏程[1] 刘昀阁 朱立贤[1] 韩广星 仝林 肖阳 张一敏[1] YUAN Yini;ZHOU Guanghui;DONG Pengcheng;LIU Yunge;ZHU Lixian;HAN Guangxing;TONG Lin;XIAO Yang;ZHANG Yimin(College of Food Science and Engineering,Shandong Agricultural University,Tai’an 271018,China;National Beef Cattle Industrial Technology System,Linyi Station,Linyi 276000,China;National Beef Cattle Industrial Technology System,Tongliao Station,Tongliao 028000,China;National Beef Cattle Industrial Technology System,Baoding Station,Baoding 071000,China)

机构地区：[1]山东农业大学食品科学与工程学院,山东泰安271018 [2]国家肉牛牦牛产业技术体系临沂站,山东临沂276000 [3]国家肉牛牦牛产业技术体系通辽站,内蒙古通辽028000 [4]国家肉牛牦牛产业技术体系保定站,河北保定071000

出　　处：《食品科学》2024年第22期291-299,共9页Food Science

基　　金：现代农业(肉牛牦牛)产业技术体系建设专项资金项目(CARS-37);山东省现代农业产业技术体系创新团队建设专项资金项目(SDAIT-09-09);国家自然科学基金青年科学基金项目(32302181)。

摘　　要：作为生鲜肉中的优势腐败菌,假单胞菌对肉品质量与安全构成严重威胁,其形成的生物被膜能够增强抗逆性,并发挥生物保护作用,从而使假单胞菌的消减更加具有挑战性。近年来,关于假单胞菌生物被膜形成机制的研究颇多,其中群体感应(quorum sensing,QS)系统和环二鸟苷酸(cyclic di-guanosine monophosphate,c-di-GMP)对生物被膜的调控作用一直是研究的焦点,并且二者之间可能存在复杂的调控关系。此外,相较于传统的生物被膜抑制策略,开发基于假单胞菌QS系统和c-di-GMP以及二者关系的抑制策略针对性更强且更具现实意义。因此,本文综述QS系统和c-di-GMP信号分子的合成过程,并从QS系统、c-di-GMP以及二者关系的角度阐述假单胞菌生物被膜形成机制,并总结针对QS系统和c-di-GMP的靶向抑制策略,旨在为食品工业中控制假单胞菌生物被膜提供理论依据。As the dominant spoilage bacterium in meat,Pseudomonas poses a significant threat to meat quality and safety.The biofilm of Pseudomonas not only enhances stress resistance but also provides bio-protection,thereby making the decontamination of Pseudomonas more challenging.In recent years,many studies have been conducted on the mechanism of Pseudomonas biofilm formation,with particular emphasis on the regulatory effect of quorum sensing(QS)and cyclic di-guanosine monophosphate(c-di-GMP)on the mechanism of Pseudomonas biofilm formation.There may be a complex interaction between QS and c-di-GMP.In addition,compared with traditional biofilm inhibition strategies,the development of new inhibition strategies based on the QS system,c-di-GMP and their relationship,which can be more targeted,has more practical significance.Therefore,this article describes the synthesis process of the QS system and c-di-GMP signal molecules and reviews the mechanism of Pseudomonas biofilm formation from the perspectives of the QS system,c-di-GMP and their relationship.Besides,it summarizes the inhibition strategies targeting the QS system and c-di-GMP.This review aims to provide a theoretical foundation for effectively controlling Pseudomonas biofilm in the food industry.

关 键 词：假单胞菌 生物被膜 群体感应系统 环二鸟苷酸 肉品安全 

分 类 号：TS201.3[轻工技术与工程—食品科学]