黑芝麻花水提液抗氧化活性及其黄酮类化合物分析  

Antioxidant activity and flavonoids analysis of water extract of black sesame flower

作　　者：廖文霞 李昌[1] LIAO Wen-Xia;LI Chang(State Key Laboratory of Food Science and Resources,Nanchang University,Nanchang 330047,China)

机构地区：[1]南昌大学,食品科学与资源挖掘全国重点实验室,南昌330047

出　　处：《食品安全质量检测学报》2024年第19期146-154,共9页Journal of Food Safety and Quality

基　　金：江西赣鄱俊才支持计划·高层次高技能领军人才培养项目(2023)。

摘　　要：目的探究不同固液比的黑芝麻花水提液的抗氧化能力和黄酮含量,鉴定黑芝麻花中所含的黄酮种类。方法以黑芝麻花为原料,分别测定不同固液比的黑芝麻花水提液的黄酮含量、1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除率和2,2’-联氮-二(3-乙基-苯并噻唑啉-6-磺酸)二铵盐[2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)ammonium salt,ABTS]阳离子自由基清除率,并对其抗氧化能力和黄酮含量进行相关性分析。同时采用超高效液相色谱-四级杆飞行时间串联质谱法(ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry,UPLC-Q-TOF-MS/MS)对黑芝麻花的黄酮类物质组成进行定性分析。结果随着固液比的增大,黑芝麻花水提液的DPPH自由基清除率和ABTS阳离子自由基清除率逐渐上升,黄酮含量也不断增高。当固液比由1:250增大到1:100时,DPPH自由基清除率从27.20%上升到61.68%,ABTS阳离子自由基清除率从28.98%上升到68.31%,黄酮含量从0.287 mg芦丁/g增加到0.631 mg芦丁/g。DPPH自由基清除率和ABTS阳离子自由基清除率呈显著正相关(P<0.01),黄酮含量与DPPH自由基清除率、ABTS阳离子自由基清除率呈显著正相关(P<0.01);并在黑芝麻花中鉴定出了8种黄酮类化合物,分别为异鼠李素-3-O-葡萄糖苷、芦丁、毛蕊花糖苷、漆黄素、山柰酚-3-O-葡萄糖醛酸苷、pratensein-7-O-glucoside、芹菜素、黄芩苷。结论黑芝麻花水提液具有良好的抗氧化活性,不同固液比的水提液表现出不同的黄酮含量和抗氧化能力。黄酮作为黑芝麻花的生理活性成分之一,具有一定的应用价值和开发前景,本研究在黑芝麻花中鉴定到了8种黄酮类化合物,不仅丰富了芝麻花中黄酮类化合物种类,还为黑芝麻花的功能评价和开发利用提供参考。Objective To investigate the antioxidant capacity and content of flavonoids of water extract in black sesame flower with different ratio of solid-liquid,and identify the types of flavonoids in black sesame flower.Methods Using black sesame flower as raw material,content of flavonoids,scavenging rate of 1,1-diphenyl-2-picrylhydrazyl(DPPH)free radical and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)ammonium salt(ABTS)cationic free radical of water extract in black sesame flower with different ratio of solid-liquid were detected,and the correlation between its antioxidant capacity and content of flavonoids was analyzed.Meanwhile,ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS/MS)was used to qualitatively analyze composition of flavonoids in black sesame flower.Results With the increase of the ratio of solid-liquid,the scavenging rate of DPPH free radical and ABTS cationic free radical of water extract in black sesame flower gradually increased,content of flavonoids also increased,when the ratio of solid-liquid increase from 1:250 to 1:100,the scavenging rate of DPPH free radical increased from 27.20%to 61.68%,the scavenging rate of ABTS cationic free radical increased from 28.98%to 68.31%,and the flavonoid content increased from 0.287 mg rutin/g to 0.631 mg rutin/g.There was a significant positive correlation between the scavenging rate of DPPH free radical and ABTS cationic free radical(P<0.01),and the content of flavonoids was significantly positively correlated with the scavenging rate of DPPH free radical and ABTS cationic free radical(P<0.01).The 8 kinds of flavonoids were identified in black sesame flower,including isorhamnetin-3-O-glucoside,rutin,verbascoside,fisetin,kaempferol-3-O-glucuronside,pratensein-7-O-glucoside,apigenin,baicalin.Conclusion The water extract of black sesame flower has good antioxidant activity,and the water extract with different ratio of solid-liquid shows different the content of flavonoids and antioxidant cap

关 键 词：黑芝麻花 抗氧化 自由基 黄酮 飞行时间质谱 

分 类 号：TS201.2[轻工技术与工程—食品科学]