DTAB反胶束萃取花椒籽中蛋白质和油脂研究  

Research on Extraction of Protein and Oil from Zanthoxylum bungeanum Maxim.Seed by DTAB Reverse Micelle

作　　者：郑永军[1,2] 王建广 郑勇 罗峰[1] ZHENG Yong-jun;WANG Jian-guang;ZHENG Yong;LUO Feng(College of Chemical and Environmental Engineering,Anyang Institute of Technology,Anyang 455000,China;Henan Key Laboratory of Subcritical High-efficiency Extraction,Anyang 455000,China)

机构地区：[1]安阳工学院化学与环境工程学院,河南安阳455000 [2]河南省亚临界高效萃取重点实验室,河南安阳455000

出　　处：《中国调味品》2024年第11期71-74,119,共5页China Condiment

基　　金：安阳工学院科研项目培育基金(YPY2021012);河南省高等学校重点科研项目(22B530001);安阳市科技攻关项目(2022C01NY002)。

摘　　要：利用十二烷基三甲基溴化铵(dodecyl trimethyl ammonium bromide,DTAB)-异辛烷-正己醇反胶束体系,对花椒籽中的蛋白质和油脂同步萃取进行了探究。结果表明,前萃过程油脂直接溶解于有机相中,蛋白质增溶于反胶束极性核中,蛋白质的前萃率受DTAB浓度、萃取时间、缓冲溶液pH值、氯化钠浓度、料液比的影响。随后,反胶束溶液通过加入等体积含有一定离子强度和pH值的水相构筑WinsorⅡ微乳体系来实现蛋白质转移。其蛋白质后萃率和油脂萃取率受后萃水相的酸度和离子强度等因素的影响。By using dodecyl trimethyl ammonium bromide(DTAB)-isooctane-n-hexanol reverse micelle system,the simultaneous extraction of protein and oil from Zanthoxylum bungeanum Maxim.seed is investigated.The results show that in the pre-extraction process,the oil is directly dissolved in the organic phase,while the protein is solubilized in the polar nucleus of the reverse micelle.The pre-extraction rate of protein is affected by the concentration of DTAB,extraction time,pH value of the buffer solution,the concentration of sodium chloride and solid-liquid ratio.Subsequently,the reverse micelle solution is used to construct WinsorⅡmicroemulsion system by adding an equal volume of aqueous phase containing a certain ionic strength and pH value to achieve protein transfer.The post-extraction rate of protein and the extraction rate of oil are influenced by the acidity and ionic strength of the aqueous phase.

关 键 词：反胶束 花椒籽 十二烷基三甲基溴化铵(DTAB) 萃取 蛋白质和油脂 

分 类 号：TS201.1[轻工技术与工程—食品科学]