Transfection of 12/15-lipoxygenase effectively alleviates inflammatory responses during experimental acute pancreatitis  

作　　者：Hong-Wei Sun Yong-Yu Bai Zhen-Liu Qin Ri-Zhao Li Tinotenda Blessing Madzikatire Percy David Papa Akuetteh Qiang Li Hong-Ru Kong Yue-Peng Jin 

机构地区：[1]National Key Clinical Specialty(General Surgery),The First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325000,Zhejiang Province,China [2]Wenzhou Medical University,Wenzhou 325000,Zhejiang Province,China

出　　处：《World Journal of Gastroenterology》2024年第42期4544-4556,共13页世界胃肠病学杂志（英文）

基　　金：National Health Commission Research Fund,No.WKJ-ZJ-2342;National Natural Science Foundation of China,No.81900583;Science and Technology Plan Project of Wenzhou,No.Y20180103.

摘　　要：BACKGROUND Acute pancreatitis(AP),the initially triggered inflammatory process in the pancreas,can be life-threatening.It has been reported that 15-lipoxygenase may promote the removal of damaged intracellular components,maintain intracellular homeostasis,and promote apoptosis by upregulating the activity of caspases.Despite an increased understanding of the lipoxygenase pathway in inflammation and immune diseases,the role of the Alox15 gene product in modulating the inflammatory changes during AP is not well defined.AIM To investigate the effect of Alox15 expression in cerulein-induced AP in rats.METHODS Model rats were transfected with Alox15 by injecting a recombinant lentivirus vector encoding Alox15 into the left gastric artery before inducing AP.The expression of Alox15 was then assessed at the mRNA and protein levels.RESULTS Our in vivo results showed that serum amylase activity and pancreatic tissue water content were significantly reduced in Alox15-transfected rats.Further,the mRNA expression levels of tumor necrosis factor alpha,interleukin(IL)-1β,IL-6,and monocyte chemoattractant protein-1,as well as the protein expression of nuclear factor kappa B in pancreatic tissue were reduced.Additionally,we observed an upregulation of cleaved caspase-3 that implies an induction of apoptosis in pancreatic cells.The transfection of Alox15 resulted in a lower number of autophagic vacuoles in AP.CONCLUSION Our findings demonstrate a regulatory role of Alox15 in apoptosis and autophagy,making it a potential therapeutic target for AP.

关 键 词：Acute pancreatitis CERULEIN 12/15-lipoxygenase APOPTOSIS AUTOPHAGY 

分 类 号：R576[医药卫生—消化系统]