上海市普陀区食品中单核细胞增生李斯特氏菌的监测与分析  

Monitoring and analysis of Listeria monocytogenes in food in Putuo District,Shanghai

作　　者：黄晶晶 顾文超[1] 汪怡 张磊[1] 吴婷婷 唐海丰[1] HUANG Jingjing;GU Wenchao;WANG Yi;ZHANG Lei;WU Tingting;TANG Haifeng(Department of Microbial Inspection,Shanghai Putuo District Center for Disease Control and Prevention,Shanghai 200333,China)

机构地区：[1]上海市普陀区疾病预防控制中心微生物检验科,上海200333

出　　处：《现代医药卫生》2024年第21期3715-3718,3725,共5页Journal of Modern Medicine & Health

基　　金：上海市加强公共卫生体系建设三年行动计划项目(GWVI-3)。

摘　　要：目的了解上海市普陀区食品中单核细胞增生李斯特氏菌(LM)的污染状况、分子分型特征及耐药性。方法选取2021—2023年从上海市普陀区各采购渠道所采集的8类食品,共计504件,对其LM阳性率进行检测,对分离鉴定所得的阳性菌株进行分子分型,并进行药物敏感性试验。结果504件样品中,共有22件样品检出LM,总检出率为4.4%,其中调理肉制品检出率最高(9.1%)。22株LM获得18个带型,带型相似度为42.11%~100.00%。22株LM对头孢西丁均耐药,部分菌株对克林霉素、苯唑西林、氨苄西林、红霉素、四环素耐药,耐药率分别为27.3%、18.2%、4.5%、4.5%、4.5%,多重耐药率为36.4%。结论上海市普陀区的肉与肉制品受LM污染最为严重,其中菌株PFGE带型呈多样性,且多重耐药比例较高。Objective To understand the contamination status,molecular typing characteristics,and drug resistance of Listeria monocytogenes(LM)in food in Putuo District,Shanghai.Methods A total of 504 samples of 8 types of food collected from various procurement channels in Putuo District,Shanghai from 2021 to 2023 were selected,and their LM positivity rate was tested.Molecular typing will be performed on the positive strains isolated and identified,and drug sensitivity tests will be conducted.Results Out of 504 samples,LM was detected in 22 samples,with a total detection rate of 4.4%,of which the detection rate of conditioned meat products was the highest(9.1%).22 LM strains obtained 18 banding patterns,with a band similarity of 42.11%-100.00%.22 strains of LM were resistant to cefoxitin,and some strains were resistant to clindamycin,penicillin,ampicillin,erythromycin,tetracycline,with resistance rates of 27.3%,18.2%,4.5%,4.5%,and 4.5%,respectively.The multidrug resistance rate was 36.4%.Conclusion Meat and meat products in Putuo District,Shanghai are most severely contaminated by LM,with a diverse range of PFGE strains and a high proportion of multi drug resistance.

关 键 词：食品 单核细胞增生李斯特氏菌 病原菌 耐药性 

分 类 号：R155.31[医药卫生—营养与食品卫生学]