基于分子生物学方法的食品中肠出血性大肠杆菌O157∶H7检测技术研究进展  

Research progress on detection technology of enterohemorrhagic Escherichia coli O157∶H7 in food based on molecular biological methods

作　　者：孙千雪 周炳武 王婷[1] 蔡琳雅 胡珊珊[1] 刘晔 SUN Qian-xue;ZHOU Bing-wu;WANG Ting;CAI Lin-ya;HU Shan-shan;LIU Ye(Department of Science and Technology,Hebei Agricultural University,Cangzhou 061000,Hebei,China;College of Food Science and Engineering,Tianjin University of Science&Technology,Tianjin 300457,China)

机构地区：[1]河北农业大学理工系,河北沧州061000 [2]天津科技大学食品科学与工程学院,天津300457

出　　处：《粮食与油脂》2024年第11期6-13,共8页Cereals & Oils

摘　　要：对检测大肠杆菌O157∶H7(E.coli O157∶H7)的预富集技术和聚合酶链式反应(PCR)技术、等温扩增技术、基于成簇规律间隔短回文重复序列及相关蛋白质系统(CRISPR/Cas)的技术等分子生物学方法进行论述,介绍了这些方法在E.coli O157∶H7检测中的研究进展,并对其检出限和靶基因等进行了比较,以期为E.coli O157∶H7的快速检测提供参考依据。Molecular biological detection methods of Escherichia coli O157∶H7(E.coli O157∶H7),such as preenrichment technology,polymerase chain reaction(PCR)technology,isothermal amplification technology,technology based on clustering regular interval short palindrome repeat and related protein system(CRISPR/Cas)were discussed.The research progress of these methods in the detection of E.coli O157∶H7 was introduced,and the detection limit and target genes were compared,in order to provide reference for the rapid detection of E.coli O157∶H7.

关 键 词：食源性致病菌 大肠杆菌O157∶H7 分子生物学检测方法 

分 类 号：TS207.4[轻工技术与工程—食品科学]