Gene expression analysis of cytokines and MMPs in melatonin and rhBMP-2 enhanced bone remodeling  

作　　者：Marina Ribeiro Paulini Letícia Ferreira Montarele Dimitrius Leonardo Pitol Gisele Giannocco Bruno Fiorelini Pereira Daniela Vieira Buchaim Carlos Henrique Bertoni Reis Rogério Leone Buchaim Joao Paulo Mardegan Issa 

机构地区：[1]Department of Basic and Oral Biology,University of São Paulo,Ribeirao Preto 14040-904,Brazil [2]Instituto de Ciências Ambientais,Químicas e Farmacêuticas,Federal University of São Paulo,Diadema 09972-270,Brazil [3]Medical School,University Center of Adamantina,Adamantina 17800-000,Brazil [4]Postgraduate Program in Structural and Functional Interactions in Rehabilitation,University of Marilia,Marilia 17525-902,Brazil [5]Department of Biological Sciences,Bauru School of Dentistry,University of São Paulo,Bauru 17012-901,Brazil [6]Department of Basic and Oral Biology,Ribeirão Preto School of Dentistry,University of São Paulo,Ribeirao Preto 14040-904,Brazil

出　　处：《World Journal of Orthopedics》2024年第11期1075-1087,共13页世界骨科杂志（英文）

摘　　要：BACKGROUND In the medical and dental fields,there is a need for studies of new therapeutic approaches for the treatment of bone defects that cause extensive bone loss.Melatonin may be an important endogenous biological factor for bone remodeling,and growth factors may enhance the repair process.AIM To evaluate the gene expression of cytokines(IL-1β,IL-6,IL-10 and TNF-α),markers of osteoclastogenesis(RANK,RANKL and OPG)and MMPs(MMP-1,MMP-2,MMP-8 and MMP-13)from the treatment of melatonin associated with an osteogenic membrane and rhBMP-2 on the recovery of a bone injury.METHODS Sixty-four rats were used and divided into 9 experimental groups and were formed according to the treatment carried out in the region of the bone lesion,which varied between the combination of 1,10 and 100μmol/L of melatonin.Gene Expression analysis was performed using real time-PCR by reading the concentration of total RNA and reverse transcription.RESULTS There were differences between groups when compared with clot or scaffold control,and improvement with a higher concentration of melatonin or rhBMP-2.The combination melatonin(1μg)with 5μg of rhBMP-2,using the guided bone regeneration technique,demonstrated some effects,albeit mild,on bone repair of critical bone defects.CONCLUSION This indicates that the approach for administering these substances needs to be reassessed,with the goal of ensuring their direct application to the affected area.Therefore,future research must be carried out,seeking to produce materials with these ideal characteristics.

关 键 词：Bone repair MELATONIN Gene expression RHBMP-2 SCAFFOLD Tissue engineering Guided bone regeneration 

分 类 号：R68[医药卫生—骨科学]