水产品中偶氮染料刚果红检测及其生物降解的研究  

Study on the detection and biodegradation of azo dye Congo red in aquatic products

作　　者：裴纪可 张传智 PEI Ji-Ke;ZHANG Chuan-Zhi(Jilin Province Medical Secondary Vocational School,Changchun 130031,China;College of Life Science,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉林省医药中等职业学校,长春130031 [2]吉林农业大学生命科学学院,长春130118

出　　处：《食品安全质量检测学报》2024年第20期52-60,共9页Journal of Food Safety and Quality

基　　金：吉林省科技发展计划项目(20230203162SF)。

摘　　要：目的检测刚果红染料污染的水体环境中,鱼、虾及贝壳类水产品体内刚果红的富集含量,并应用生物降解方式实现水体中刚果红的高效降解。方法构建刚果红污染养殖水体模型,选择鲫鱼、青虾、花蛤、蛏子作为研究对象,检测内脏及肌肉组织中刚果红含量的差异。利用布丘氏菌(Buttiauxella)S19-1降解养殖水中的刚果红,考察不同培养条件对刚果红降解率的影响,采用气相色谱-质谱仪(gas chromatography-mass spectrometer,GC-MS)检测刚果红降解产物。结果4种水产品体内刚果红含量大小排序为蛏子>花蛤>青虾>鲫鱼,鱼、虾类内脏中的刚果红浓度分别为鱼肉和虾肉的含量的2.7~3.5倍和3.3~5.4倍。利用S19-1菌株对养殖水进行生物降解,在降解温度32℃、降解时间60 h、pH为7、盐浓度为5%、刚果红质量浓度为100 mg/L的条件下,降解率最高可达到82.7%;通过GC-MS检测刚果红降解产物,共发现6种代谢产物。结论蛏子、花蛤、青虾、鲫鱼对刚果红具有富集作用,S19-1菌株能够降解污染水体中的刚果红,研究成果为偶氮染料的生物降解提供理论基础和技术手段。Objective To detect the accumulation levels of Congo red in fish,shrimp,and shellfish in Congo red dye-polluted aquatic environments and to achieve efficient degradation of Congo red in water through biodegradation.Methods A model of Congo red-polluted aquaculture water was constructed,and crucian carp,shrimp,clams,and razor clams were selected as the research objects to detect the differences in Congo red content in internal organs and muscle tissues.Buttiauxella S19-1 was used to degrade Congo red in aquaculture water,and the effects of different culture conditions on the degradation rate of Congo red were investigated.The degradation products were analyzed using gas chromatography-mass spectrometer(GC-MS).Results The size ranking of Congo red content in 4 kinds of aquatic products was razor clam>clam>shrimp>crucian carp.The concentration of Congo red in the viscera of fish and shrimp was 2.7-3.5 times and 3.3-5.4 times higher,respectively than in fish and shrimp muscle.The biodegradation of aquaculture water using strain S19-1 achieved a maximum degradation rate of 82.7%under optimal conditions,including a degradation temperature of 32℃,degradation time of 60 hours,pH 7,salt concentration of 5%,and an initial Congo red concentration of 100 mg/L.Six kinds of degradation products were identified via GC-MS analysis.Conclusion Razor clams,clams,shrimp,and crucian carp show bioaccumulation of Congo red.The S19-1 strain can degrade Congo red in contaminated water,providing a theoretical basis and technical means for the biodegradation of azo dyes.

关 键 词：水产品 水污染 刚果红 生物富集 生物降解 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程] O657.63[轻工技术与工程—食品科学与工程]