裸藻多糖超声碱提工艺优化、结构表征及抗氧化活性研究  

Ultrasonic-alkaline extraction,characterization,and antioxidant activity of paramylon from Euglena gracilis

作　　者：胡锋阳 李市场[1,2] 宋根娣 丁慧 常英杰 龚明贵 HU Fengyang;LI Shichang;SONG Gendi;DING Hui;CHANG Yingjie;GONG Minggui(College of Food and Bioengineering,Henan University of Science and Technology,Luoyang 471023,China;Henan Provincial Food Microbiology Engineering Technology Research Center,Luoyang 471023,China;Department of Environmental Engineering and Chemistry,Luoyang Institute of Science and Technology,Luoyang 471023,China;Luoyang Ouke Baike Biotechnology Co.Ltd.,Luoyang 471023,China)

机构地区：[1]河南科技大学食品与生物工程学院,河南洛阳471023 [2]河南省食品微生物工程技术研究中心,河南洛阳471023 [3]洛阳理工学院环境工程与化学学院,河南洛阳471023 [4]洛阳欧科拜克生物技术股份有限公司,河南洛阳471023

出　　处：《食品与发酵工业》2024年第22期28-36,共9页Food and Fermentation Industries

基　　金：国家自然科学基金项目(31900005);国家自然科学基金面上项目(31870093)。

摘　　要：该文探究了裸藻多糖(Euglena gracilis polysaccharide,EGP)的提取率、结构表征及体外抗氧化活性。采用超声碱提工艺结合Plackett-Burman和响应面设计对EGP提取率进行优化,采用凝胶渗透色谱、离子色谱、傅里叶变换红外光谱、扫描电镜和自由基清除实验对EGP的分子质量、单糖组成、基团构成、微观结构和抗氧化活性进行分析。超声碱提法用于EGP提取的最佳工艺参数为料液比为1∶40(g∶mL),水浴温度为41℃,水浴时间为0.9 h,NaOH溶液浓度为1 mol/L,超声功率为120 W,超声时间为3 min,在此条件下,EGP的提取率为(62.84±0.49)%。结果表明,EGP由100%葡萄糖组成,通过β-糖苷键连接,分子质量为2.13×10^(4)Da。抗氧化实验结果表明,EGP对羟自由基、DPPH自由基、ABTS阳离子自由基、超氧阴离子自由基均表现出良好的抗氧化活性。EGP的结构研究为裸藻的继续开发利用提供了科学的理论依据。Euglena gracilis polysaccharide(EGP),as a new natural functional food,has attracted more and more attention.This study investigated the extraction yield,structural characterization,and in vitro antioxidant activity of EGP.The extraction yield EGP was optimized with ultrasonic-alkali extraction by Plackett-Burman and response surface design.The molecular weight,monosaccharide composition,group composition,microstructure,and antioxidant activity of EGP were analyzed using gel permeation chromatography(GPC),ion chromatography(IC),Fourier transform infrared spectroscopy(FT-IR),scanning electron microscope(SEM),and free radical scavenging tests.The optimum process conditions were as follows:the liquid-to-material ratio of 1∶40(g∶mL),water bath temperature of 41℃,water bath time of 0.9 h,NaOH solution concentration of 1 mol/L,ultrasonic power of 120 W,and ultrasonic time of 3 min.Under these conditions,the extraction yield was(62.84±0.49)%.Results indicated that EGP was composed of 100%glucose and connected byβ-glycosidic bonds,and the molecular weight were 2.13×10^(4)Da.Antioxidant experiments demonstrated that EGP showed good antioxidant activity against·OH,DPPH free radicals,ABTS cationic radicals,and·O-2.The structural study of EGP provides a scientific theoretical basis for the continued development and utilization of Euglena gracilis.

关 键 词：裸藻 裸藻多糖 响应面法 结构表征 抗氧化活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]