基于稀酸预处理和库德毕赤酵母发酵的蜈蚣藻多糖利用制备生物乙醇研究  

Production of bioethanol from polysaccharides in Grateloupia livida based on dilute acid pretreatment and Pichia kudriavzevii fermentation

作　　者：丘仪 杨贤庆[2] 李春生[2] 戚勃[2] 赵永强[2] 潘创 陈胜军[2] QIU Yi;YANG Xianqing;LI Chunsheng;QI Bo;ZHAO Yongqiang;PAN Chuang;CHEN Shengjun(College of Food Science and Technology,Guangdong Ocean University,Zhanjiang 524088,China;Key Laboratory of Aquatic Products Processing,Ministry of Agriculture and Rural Affairs,South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China)

机构地区：[1]广东海洋大学食品科技学院,广东湛江524088 [2]中国水产科学研究院南海水产研究所,农业农村部水产品加工重点实验室,广东广州510300

出　　处：《食品与发酵工业》2024年第22期159-166,共8页Food and Fermentation Industries

基　　金：国家藻类产业技术体系(CARS-50);中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金(2023TD78)。

摘　　要：蜈蚣藻多糖含量丰富,价格低廉,具有发酵生产生物乙醇的潜力。该研究以蜈蚣藻为原料,通过单因素和响应面试验优化确定最佳稀酸预处理蜈蚣藻水解液的条件为料液比1∶20(g∶mL)、时间1.8 h、温度100℃、稀硫酸质量分数为3%,获得的还原糖含量最高,达到37.64%。对稀酸预处理蜈蚣藻水解液中单糖组成和含量进行分析,结果发现,稀酸预处理能够显著提高蜈蚣藻水解液中单糖含量,半乳糖和葡萄糖是其中含量最高的单糖组分。利用库德毕赤酵母对蜈蚣藻水解液进行发酵,结果发现该菌具有良好的生长和产乙醇能力,而且接种量的增加能够显著提高菌株的生物量和乙醇发酵特性。Pearson相关性分析显示,蜈蚣藻水解液中各单糖含量与生物量和各乙醇发酵参数呈显著负相关,生物量与各乙醇发酵参数呈显著正相关,表明蜈蚣藻水解液中单糖的消耗在库德毕赤酵母的生长和乙醇生产过程中起重要作用,而酵母菌的生长状况直接关系到乙醇的发酵生产。相关性网络图显示,葡萄糖和半乳糖的消耗对库德毕赤酵母生物量增加和乙醇生产的影响最大。结果表明,利用蜈蚣藻为原料通过稀酸预处理结合库德毕赤酵母发酵,具有生产生物乙醇的应用潜力,该研究也为其他海藻发酵生产生物乙醇提供重要的技术参考。Fermentation of Grateloupia livida has the potential to produce bioethanol because it is rich in polysaccharides and low price.In this study,G.livida was used as raw material for bioethanol production.The optimal pretreatment condition for hydrolysis of G.livida by dilute sulfuric acid was obtained through the single factor and response surface test,including the solid-liquid ratio of 1∶20(g∶mL),hydrolysis time of 1.8 h,hydrolysis temperature of 100℃,and sulfuric acid mass fraction of 3%,in which condition the reducing sugar content reached the maximum(37.64%).The compositions and contents of monosaccharides in the hydrolysate of G.livida pretreated with dilute sulfuric acid were analyzed.Results showed that dilute sulfuric acid pretreatment could significantly increase the content of monosaccharides,among which galactose and glucose possessed the highest contents.The hydrolysate of G.livida was further fermented by Pichia kudriavzevii.This yeast exhibited good growth and ethanol production ability,and the increase of inoculation could significantly enhance its biomass and ethanol fermentation characteristics.Pearson correlation analysis showed that the contents of monosaccharides in the hydrolysate of G.livida were significantly negatively correlated with biomass and various ethanol fermentation parameters,while the biomass was significantly positively correlated with various ethanol fermentation parameters.It indicated that the consumption of monosaccharides played an important role in the growth and ethanol production of P.kudriavzevii,and the growth of yeast was directly related to the production of ethanol fermentation.The correlation network showed that the consumption of glucose and galactose had the greatest influence on the biomass increase and ethanol production of P.kudriavzevii.These results suggest that the use of G.livida as raw material through dilute acid pretreatment combined with P.kudriavzevii fermentation has an application potential to produce bioethanol,which also provides an important

关 键 词：蜈蚣藻 酸水解 生物乙醇 库德毕赤酵母 还原糖含量 单糖组成 相关性网络 

分 类 号：TQ223.122[化学工程—有机化工] TQ920.6[轻工技术与工程—发酵工程]