木聚糖钙、铁、锌配合物的理化性质及其协同益生作用  

Physicochemical properties of xylan,calcium,iron,and zinc complexes and their synergistic probiotic effects

作　　者：张绮颖 蔡悦蓓 董宝锐 姜铁民 周玉恒 李霞[1] ZHANG Qiying;CAI Yuebei;DONG Baorui;JIANG Tiemin;ZHOU Yuheng;LI Xia(College of Chemistry and Bioengineering,Guilin University of Science andTechnology,Guilin 541004,China;Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization,Guangxi Institute of Botany,Chinese Academy of Sciences,Guilin 541006,China)

机构地区：[1]桂林理工大学化学与生物工程学院,广西桂林541004 [2]广西植物功能物质研究与利用重点实验室,中国科学院广西植物所,广西桂林541006

出　　处：《食品与发酵工业》2024年第22期167-175,共9页Food and Fermentation Industries

基　　金：广西自然科学基金项目(2021GXNSFAA220072);中央引导地方科技发展专项资金项目(桂科ZY22096025)。

摘　　要：以木聚糖(xylan,Xyl)为原料,采用金属元素修饰的化学合成法制备木聚糖钙、铁、锌配合物,通过对金属元素含量、灰分含量、基本化学成分、分子质量、单糖组成、粒径和黏度的测定确定其理化性质,利用响应面优化法研究其协同益生作用。结果表明,木聚糖钙配合物(xylan-calcium complex,Xyl-Ca)的Ca含量为(7.57±0.03)mg/g,木聚糖铁配合物(xylan-iron complex,Xyl-Fe)的Fe含量为(6.26±0.06)mg/g,木聚糖锌配合物(xylan-zinc complex,Xyl-Zn)的Zn含量为(3.21±0.02)mg/g;金属络合可降低Xyl的糖含量,引起单糖组成含量的差异;木聚糖及其金属配合物的灰分含量、分子质量、粒径和黏度大小排序均为:Xyl-Fe>Xyl-Ca>Xyl-Zn>Xyl;响应面法得到复合木聚糖金属配合物对三联益生菌促增殖作用的最佳质量配比为:Xyl-Ca∶Xyl-Fe∶Xyl-Zn=8.9∶9.8∶11.3,在此配比浓度下培养液的OD值为1.1255。所获结果表明,Xyl-Ca、Xyl-Fe、Xyl-Zn可用作功能性食品和药物应用的潜在钙铁锌补充剂,是改善肠道健康或治疗肠道疾病的益生元替代衍生物。Xylan(Xyl)were used as raw materials to prepare xylan-mineral complexes by chemical synthesis modified by metal elements,and their physical and chemical properties were determined by the determination of metal element content,ash content,basic chemical composition,molecular weight,monosaccharide composition,particle size and viscosity,and their synergistic probiotics were studied by response surface optimization method.Results showed that the Ca content of the xylan-calcium complex(Xyl-Ca)was(7.57±0.03)mg/g,the Fe content of the xylan-iron complex(Xyl-Fe)was(6.26±0.06)mg/g,and the Zn content of the xylan-zinc complex(Xyl-Zn)was(3.21±0.02)mg/g.Metal complexation could reduce the sugar content of Xyl and cause differences in the composition of monosaccharides.The ash content,molecular weight,particle size,and viscosity of xylan and its metal complexes were in the following order:Xyl-Fe>Xyl-Ca>Xyl-Zn>Xyl.The optimal weight ratio of the composite xylan metal complex on the proliferation promotion of triple probiotics was Xyl-Ca∶Xyl-Fe∶Xyl-Zn=8.9∶9.8∶11.3,and the OD value of the culture medium was 1.1255.Results suggested that Xyl-Ca,Xyl-Fe,and Xyl-Zn could be used as potential calcium-iron-zinc supplements for functional food and pharmaceutical applications,and were alternative derivatives of prebiotics for improving intestinal health or treating intestinal diseases.

关 键 词：木聚糖 多糖金属配合物 三联益生菌 益生元 协同益生作用 

分 类 号：TS201.3[轻工技术与工程—食品科学] TS201.2[轻工技术与工程—食品科学与工程]