雨生红球藻藻渣多糖提取条件优化及降血脂活性分析  

Extraction Condition Optimization and Blood Lipid-Lowering Activity of Polysaccharide from Haematococcus pluvialis Residue

作　　者：藏颖 梁钟文 刘松 高丹 黄羽诺 李媚 刘红全 ZANG Ying;LIANG Zhongwen;LIU Song;GAO Dan;HUANG Yunuo;LI Mei;LIU Hongquan(Guangxi Key Laboratory for Polysaccharide Materials and Modification,College of Marine and Biotechnology,Guangxi Minzu University,Nanning 530007,Guangxi,China)

机构地区：[1]广西民族大学海洋与生物技术学院广西多糖材料与改性重点实验室,广西南宁530007

出　　处：《食品研究与开发》2024年第22期102-110,共9页Food Research and Development

基　　金：广西自然科学基金项目(2018GXNSFAA294032、2023GXNSFAA026409)。

摘　　要：为提高雨生红球藻藻渣多糖的提取率,采用单因素和响应面法优化雨生红球藻藻渣多糖提取条件,并对获得的多糖进行降血脂试验。结果表明,雨生红球藻藻渣多糖最佳提取条件为NaOH质量分数2.5%、料液比1∶25(g/mL)、超声功率250 W、超声时间10 min、提取时间2.5 h,在此条件下,多糖的提取率为(5.744±0.030)%。藻渣多糖浓度为5 mg/mL时,对胰脂肪酶有较好的抑制效果,抑制率达到(94.19±0.02)%。The extraction conditions of polysaccharide from the residue of Haematococcus pluvialis were opti-mized by single factor tests and response surface methodology to improve the yield of polysaccharides.Experi-ments were carried out to evaluate the blood lipid-lowering effect of the obtained polysaccharide.The extrac-tion conditions were optimized as follows:NaOH mass fraction of 2.5%,solid-to-liquid ratio of 1∶25(g/mL),ul-trasonic power of 250 W,ultrasonic time of 10 min,and extraction time of 2.5 h.Under these conditions,the yield of polysaccharide was(5.744±0.030)%.The obtained polysaccharide at 5 mg/mL inhibited the activity of pancreatic lipase,with the inhibition rate reaching(94.19±0.02)%.

关 键 词：雨生红球藻藻渣 多糖 超声辅助提取 工艺优化 降血脂 

分 类 号：TS201.4[轻工技术与工程—食品科学]