硫酸锌处理对西兰花芽苗关键活性成分影响及硫苷变化分子机制初步探讨  

Effect of Zinc Sulphate Treatments on Key Active Components and the Molecular Mechanism of Thioglycoside Changes of Broccoli Sprouts

作　　者：赵子璇 刘光敏[2] 何洪巨[2] 沈巧玲 兰慧至 王泽宇 黄伟[1] ZHAO Zixuan;LIU Guangmin;HE Hongju;SHEN Qiaoling;LAN Huizhi;WANG Zeyu;HUANG Wei(Hebei North University,Zhangjiakou 075000,China;Institute of Agri-food Processing and Nutrition,Beijing Academy of Agricultural and Forestry Sciences,Beijing 100097,China;Hebei Agricultural University,Baoding 071000,China)

机构地区：[1]河北北方学院,河北张家口075000 [2]北京市农林科学院农产品加工与食品营养研究所,北京100097 [3]河北农业大学,河北保定071000

出　　处：《食品工业科技》2024年第22期27-36,共10页Science and Technology of Food Industry

基　　金：北京市农林科学院创新能力建设专项项目(KJCX20230212,KJCX20220419);现代农业产业技术体系北京市创新团队(BAIC01-2023-22);河北省现代农业产业技术体系露地蔬菜产业创新团队建设-大宗叶菜岗位(HBCT2023110201)。

摘　　要：为探究喷施不同浓度硫酸锌对西兰花芽苗活性成分积累和抗氧化活性的影响,并从分子水平探讨硫酸锌对西兰花芽苗中硫苷的调控机制,本文以7 d西兰花芽苗为试验材料,采用不同浓度(0、1、2、4、8 mmol/L)硫酸锌(Zinc sulphate,ZnSO_(4))处理西兰花芽苗,测定西兰花芽苗叶绿素、抗坏血酸、总黄酮、总酚、硫苷含量、黑芥子酶活以及DPPH自由基清除能力,研究不同浓度ZnSO_(4)处理组对西兰花芽苗活性成分的影响,并且利用荧光定量PCR技术分析硫苷合成关键基因表达量的变化。结果表明,各处理组叶绿素含量均低于对照;抗坏血酸含量随硫酸锌浓度的增大呈上升趋势,各处理组均高于对照组。总酚含量在ZnSO_(4)浓度为8 mmol/L时含量最高且显著高于对照组(P<0.05);总黄酮、总硫苷和RAA含量、黑芥子酶活性及DPPH自由基清除率在ZnSO_(4)浓度为2 mmol/L时达最大值。在ZnSO_(4)浓度为2 mmol/L时CYP83A1、CYP79F1与FMO-GSOX2基因表达量显著提高(P<0.05);FMO-GSOX2与总硫苷、RAA、脂肪族硫苷、黑芥子酶呈极显著正相关(P<0.01);CYP79F1、CYP83A1与总硫苷、RAA、脂肪族硫苷呈显著性正相关(P<0.05)。说明FMO-GSOX2、CYP79F1和CYP83A1表达量的上调促进了总硫苷、脂肪族硫苷和RAA的合成。本研究结果表明,外源喷施适量硫酸锌能促进硫苷合成相关基因的表达,其中2 mmol/L ZnSO_(4)可有效增加西兰花芽苗中活性物质含量及抗氧化活性。To investigated the effects of spray application of zinc sulfate(ZnSO_(4))solution on the accumulation of crucial bioactive components and antioxidant activity,and explored the regulatory mechanism of zinc sulfate on glucosinolates in broccoli flower buds and seedlings at the molecular level,seven-day-old broccoli sprouts were treated with one of four concentrations(1,2,4,and 8 mmol/L)of aqueous ZnSO_(4) solutions or water(0 mmol/L ZnSO_(4) as the control).The effects of each ZnSO_(4) treatment on the bioactive components of broccoli sprouts were investigated by determining the contents of chlorophyll,ascorbic acid,total flavonoids,total phenols,and total glucosinolates,as well as myrosinase activity and DPPH free-radical scavenging rate.The expression levels of glucosinolate synthesis structural genes in the sprouts were analyzed using a real-time quantitative PCR detection system.The results showed that the chlorophyll content of all ZnSO_(4) treatments was lower than that of the control.The ascorbic acid content tended to be elevated with an increase in ZnSO_(4) concentration and was higher than that of the control for all ZnSO_(4) treatments.The total polyphenol content was the highest in response to 8 mmol/L ZnSO_(4) treatment and was significantly(P<0.05)higher than that of the control.The contents of total flavonoid content,total glucosinolates,glucoraphanin,myrosinase activity and maximum DPPH free-radical scavenging rate were greatest after treatment with 2 mmol/L ZnSO_(4) solution.The expression levels of CYP83A1,CYP79F1,and FMO-GSOX2 were significantly(P<0.05)increased in response to 2 mmol/L ZnSO_(4) treatment.Thus,spray application of exogenous ZnSO_(4) promoted the expression of crucial genes involved in thioredoxin synthesis.FMO-GSOX2 showed highly significant(P<0.01)positive correlation with total glucosinolates,RAA,aliphatic glucosinolates,and myrosinase activity.CYP79F1 and CYP83A1 showed significant(P<0.05)positive correlation with total glucosinolates,RAA,and aliphatic glucosinolates.It indica

关 键 词：西兰花芽苗 硫酸锌 硫苷 活性成分 抗氧化活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]